<p>All samples were imaged using fluorescent scanning confocal microscopy (Nikon confocal A1R, excitation 488-nm@16mW, emission filter 525/50) to evaluate fusion events. Cells were segmented using the transmitted light channel and instances of bright fluorescent spots within cells were evaluated. Image analysis was performed in ImageJ.</p>
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<divclass="h1">Data and Code</div>
<p>We are making our code and data publicly available to help future iGEM teams who wish to build on our work.</p>
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<p>See <ahref="/tu-delft/parts/">Parts and Sequences</a> for more details on the design and sequence of our parts.</p>
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<divclass="h1">Lab Notebook</div>
<p>The lab notebook can be viewed <atarget="_blank"href="https://static.igem.wiki/teams/5054/notebook-compressed.pdf">here</a>.</p>
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<divclass="h1">Protocols</div>
<divclass="h2">Yeast Transformation</div>
<p>Adapted from <ahref="#cite7">[7]</a> following <ahref="#cite8">[8]</a>.</p>
{% block lead %}Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.{% endblock %}