<p>If, like other organelles, UCYN-A relies on proteins imported from the host for normal functioning, characterizing the import system and the targeting sequence is essential before transplanting the organelle into a new host organism. Building upon the work of Coale et al. <ahref="#cite1"style="color: #185A4F;">[1]</a>, we aimed to advance the understanding of uTP by identifying its precise sequence.</p>
<p>If, like other organelles, UCYN-A relies on proteins imported from the host for normal functioning, characterizing the import system and the targeting sequence is essential before transplanting the organelle into a new host organism. Building upon the work of Coale et al. <ahref="#cite1"style="color: #185A4F;">[1]</a>, we aimed to advance the understanding of uTP by identifying its precise sequence.</p>
<p>Starting from the raw proteomics data from <ahref="#cite1"style="color: #185A4F;">[1]</a>, we selected 368 proteins expressed by the host and significantly enriched in UCYN-A and performed multiple sequence alignment (MSA). Using the alignment we identified a strongly conserved C-terminal region in many of the imported proteins similar to that reported by <ahref="#cite1"style="color: #185A4F;">[1]</a>. We selected a subset of 206 proteins with highly similar (>60% sequence identity) C-terminal alignments, indicating that these are likely to contain uTP,</p>
<p>Starting from the raw proteomics data from <ahref="#cite1"style="color: #185A4F;">[1]</a>, we selected 368 proteins expressed by the host and significantly enriched in UCYN-A and performed multiple sequence alignment (MSA). Using the alignment we identified a strongly conserved C-terminal region in many of the imported proteins similar to that reported by <ahref="#cite1"style="color: #185A4F;">[1]</a>. We selected a subset of 206 proteins with highly similar (>60% sequence identity) C-terminal alignments, indicating that these are likely to contain uTP.</p>
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<imgsrc="https://static.igem.wiki/teams/5054/msa.png"alt="Fig 1: Graphical overview of the experiment plan.">
<imgsrc="https://static.igem.wiki/teams/5054/msa.png"alt="Fig 1: Graphical overview of the experiment plan.">
<figcaption>Fig 2: Multiple sequence alignment (MSA) of all UCYN-A enriched sequences. The strongly aligned C-terminal region is highlighted (positions 880-1010 in the alignment)
<figcaption>Fig 2: Multiple sequence alignment (MSA) of all UCYN-A enriched sequences. The strongly aligned C-terminal region is highlighted (positions 880-1010 in the alignment)
