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static/pOpt2_mVenus_Ble.gb

+LOCUS       Exported                6345 bp ds-DNA    circular SYN 28-JUNI-2017
+DEFINITION  .
+ACCESSION   .
+VERSION     .
+KEYWORDS    pOpt2_YFP_Ble
+SOURCE      synthetic DNA construct
+  ORGANISM  synthetic DNA construct
+REFERENCE   1  (bases 1 to 6345)
+  AUTHORS   Thomas Baier
+  TITLE     Direct Submission
+  JOURNAL   Exported 6. Nov. 2017 from SnapGene 2.3.2
+            http://www.snapgene.com
+FEATURES             Location/Qualifiers
+     source          1..6345
+                     /organism="synthetic DNA construct"
+                     /mol_type="other DNA"
+     rep_origin      complement(24..330)
+                     /direction=LEFT
+                     /label="f1ori"
+                     /note="f1ori"
+     misc_feature    complement(360..507)
+                     /label="lacZ_a_KS"
+                     /note="lacZ_a_KS"
+     primer_bind     complement(466..485)
+                     /label="Sequencing_primer_binding_site"
+                     /note="Sequencing_primer_binding_site"
+     primer_bind     complement(474..496)
+                     /label="M13_pUC-fwd_primer"
+                     /note="M13_pUC-fwd_primer"
+     primer_bind     complement(489..505)
+                     /label="M13_forward20_primer"
+                     /note="M13_forward20_primer"
+     promoter        516..533
+                     /label="T7_promoter"
+                     /note="T7_promoter"
+     primer_bind     523..544
+                     /label="InsrtCKpJR38_Fw"
+                     /note="InsrtCKpJR38_Fw"
+     primer_bind     560..571
+                     /label="pBluescriptSK_primer"
+                     /note="pBluescriptSK_primer"
+     promoter        572..838
+                     /label="HSP70Ap"
+                     /note="HSP70Ap promoter"
+     promoter        845..1065
+                     /label="RBCS2p"
+                     /note="RBCS2p promoter"
+     transit_peptide join(1066..1073,1219)
+                     /note="RBCS2ORF"
+     intron          1074..1218
+                     /label="RBCS2i(1)"
+                     /note="RBCS2i"
+     CDS             1247..1258
+                     /codon_start=1
+                     /product="Factor Xa recognition and cleavage site"
+                     /note="Factor Xa site"
+                     /translation="IEGR"
+     CDS             join(1259..1568,1898..2301)
+                     /codon_start=1
+                     /label="mVenus"
+                     /note="mVenus"
+                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
+                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
+                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
+                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
+                     FVTAAGITLGMDELYK"
+     intron          1569..1897
+                     /label="RBSC2i2"
+                     /note="RBSC2i2"
+     CDS             2302..2313
+                     /codon_start=1
+                     /product="Factor Xa recognition and cleavage site"
+                     /note="Factor Xa site"
+                     /translation="IEGR"
+     CDS             2326..2349
+                     /codon_start=1
+                     /product="peptide that binds Strep-Tactin(R), an engineered
+                     form of streptavidin"
+                     /note="Strep-Tag II"
+                     /translation="WSHPQFEK"
+     misc_feature    2353..2586
+                     /label="3'UTR"
+                     /note="3'UTR"
+     promoter        2599..2865
+                     /label="HSP70Ap(1)"
+                     /note="HSP70Ap"
+     promoter        2872..3092
+                     /label="RBCS2p"
+                     /note="RBCS2p promoter"
+     transit_peptide join(3093..3100,3246)
+                     /note="RBCS2ORF"
+     intron          3101..3245
+                     /label="RBCS2i(1)"
+                     /note="RBCS2i"
+     CDS             join(3253..3420,3566..3778)
+                     /codon_start=1
+                     /note="ble"
+                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
+                     VTLFISAVQDQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
+                     GREFALRDPAGNCVHFVAEEQD"
+     intron          3421..3565
+                     /label="RBCS2_intron"
+                     /note="RBCS2_intron"
+     misc_feature    3788..4026
+                     /label="3'UTR(1)"
+                     /note="3'UTR"
+     primer_bind     complement(4086..4104)
+                     /label="M13_reverse_primer"
+                     /note="M13_reverse_primer"
+     primer_bind     complement(4103..4125)
+                     /label="M13_pUC_rev_primer"
+                     /note="M13_pUC_rev_primer"
+     promoter        complement(4139..4168)
+                     /label="lac_promoter"
+                     /note="lac_promoter"
+     rep_origin      complement(4477..5096)
+                     /direction=LEFT
+                     /label="pBR322ori"
+                     /note="pBR322ori"
+     CDS             complement(5245..6105)
+                     /codon_start=1
+                     /label="Ampicillin"
+                     /note="Ampicillin"
+                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
+                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
+                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
+                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
+                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
+                     LIKHW"
+     promoter        complement(6153..6180)
+                     /label="AmpRp"
+                     /note="AmpRp promoter"
+ORIGIN
+        1 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
+       61 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
+      121 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
+      181 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
+      241 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
+      301 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
+      361 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
+      421 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
+      481 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
+      541 ggagctccac cgcggtggcg gccgctctag agctgaggct tgacatgatt ggtgcgtatg
+      601 tttgtatgaa gctacaggac tgatttggcg ggctatgagg gcgggggaag ctctggaagg
+      661 gccgcgatgg ggcgcgcggc gtccagaagg cgccatacgg cccgctggcg gcacccatcc
+      721 ggtataaaag cccgcgaccc cgaacggtga cctccacttt cagcgacaaa cgagcactta
+      781 tacatacgcg actattctgc cgctatacat aaccactcag ctagcttaag atcccatcac
+      841 cggtgcatgc cgggcgcgcc agaaggagcg cagccaaacc aggatgatgt ttgatggggt
+      901 atttgagcac ttgcaaccct tatccggaag ccccctggcc cacaaaggct aggcgccaat
+      961 gcaagcagtt cgcatgcagc ccctggagcg gtgccctcct gataaaccgg ccagggggcc
+     1021 tatgttcttt acttttttac aagagaagtc actcaacatc ttaaaatggc caggtgagtc
+     1081 gacgagcaag cccggcggat caggcagcgt gcttgcagat ttgacttgca acgcccgcat
+     1141 tgtgtcgacg aaggcttttg gctcctctgt cgctgtctca agcagcatct aaccctgcgt
+     1201 cgccgtttcc atttgcagga tgcatatggg atccagatct gacgtcatcg agggcagggt
+     1261 gagcaagggc gaggagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga
+     1321 cgtgaacggc cacaagttca gcgtgagcgg cgagggcgag ggcgacgcca cctacggcaa
+     1381 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctggt
+     1441 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
+     1501 cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gagcgcacca tcttcttcaa
+     1561 ggacgacggt gagcttgcgg ggttgcgagc aacactccag caacgaacag tgcccaagtc
+     1621 aggaatctgc agtcagcctg ggctttcggc ggctttttct tgggcaaaca gcttgcactc
+     1681 atgccagcgc ggcttgtcca gcctcacttg agctttccag ctgctaccag ccgggctata
+     1741 cgacagcgac agagccatag cgtggaatca cttatttggg ttgccgaagt agcggtcgga
+     1801 gcgtgagttc ttggtcaagc cgccccttat ccggttcctg tccgtgtctt tgtccctcgt
+     1861 tcacccttcg cggcaccctt catccccttg cttgcaggta actacaagac ccgcgccgag
+     1921 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
+     1981 gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca caacgtgtac
+     2041 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
+     2101 gaggacggcg gcgtgcagct ggccgaccac taccagcaga acacccccat cggcgacggc
+     2161 cccgtgctgc tgcccgacaa ccactacctg agctaccaga gcaagctgag caaggacccc
+     2221 aacgagaagc gcgaccacat ggtgctgctg gagttcgtga ccgccgccgg catcaccctg
+     2281 ggcatggacg agctgtacaa gatcgagggc agggatatcg aattctggag ccacccgcag
+     2341 ttcgagaagt aaccgctccg tgtaaatgga ggcgctcgtt gatctgagcc ttgccccctg
+     2401 acgaacggcg gtggatggaa gatactgctc tcaagtgctg aagcggtagc ttagctcccc
+     2461 gtttcgtgct gatcagtctt tttcaacacg taaaaagcgg aggagttttg caattttgtt
+     2521 ggttgtaacg atcctccgtt gattttggcc tctttctcca tgggcgggct gggcgtattt
+     2581 gaagcgacta gtacgcgtgc tgaggcttga catgattggt gcgtatgttt gtatgaagct
+     2641 acaggactga tttggcgggc tatgagggcg ggggaagctc tggaagggcc gcgatggggc
+     2701 gcgcggcgtc cagaaggcgc catacggccc gctggcggca cccatccggt ataaaagccc
+     2761 gcgaccccga acggtgacct ccactttcag cgacaaacga gcacttatac atacgcgact
+     2821 attctgccgc tatacataac cactcagcta gcttaagatc ccatccctag ggcatgccgg
+     2881 gcgcgccaga aggagcgcag ccaaaccagg atgatgtttg atggggtatt tgagcacttg
+     2941 caacccttat ccggaagccc cctggcccac aaaggctagg cgccaatgca agcagttcgc
+     3001 atgcagcccc tggagcggtg ccctcctgat aaaccggcca gggggcctat gttctttact
+     3061 tttttacaag agaagtcact caacatctta aaatggccag gtgagtcgac gagcaagccc
+     3121 ggcggatcag gcagcgtgct tgcagatttg acttgcaacg cccgcattgt gtcgacgaag
+     3181 gcttttggct cctctgtcgc tgtctcaagc agcatctaac cctgcgtcgc cgtttccatt
+     3241 tgcaggaagc ttatggccaa gctgaccagc gccgttccgg tgctcaccgc gcgcgacgtg
+     3301 gccggagcgg tcgagttctg gaccgaccgg ctcgggttca gccgggactt cgtggaggac
+     3361 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
+     3421 gtgagtcgac gagcaagccc ggcggatcag gcagcgtgct tgcagatttg acttgcaacg
+     3481 cccgcattgt gtcgacgaag gcttttggct cctctgtcgc tgtctcaagc agcatctaac
+     3541 cctgcgtcgc cgtttccatt tgcaggacca ggtggtgccg gacaacaccc tggcctgggt
+     3601 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
+     3661 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
+     3721 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactaact
+     3781 cgagtgaccg ctccgtgtaa atggaggcgc tcgttgatct gagccttgcc ccctgacgaa
+     3841 cggcggtgga tggaagatac tgctctcaag tgctgaagcg gtagcttagc tccccgtttc
+     3901 gtgctgatca gtctttttca acacgtaaaa agcggaggag ttttgcaatt ttgttggttg
+     3961 taacgatcct ccgttgattt tggcctcttt ctccatgggc gggctgggcg tatttgaagc
+     4021 ggacccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
+     4081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
+     4141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
+     4201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
+     4261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
+     4321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
+     4381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
+     4441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
+     4501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
+     4561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
+     4621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
+     4681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
+     4741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
+     4801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
+     4861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
+     4921 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
+     4981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
+     5041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
+     5101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
+     5161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
+     5221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
+     5281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
+     5341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
+     5401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
+     5461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
+     5521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
+     5581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
+     5641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
+     5701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
+     5761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
+     5821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
+     5881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
+     5941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
+     6001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
+     6061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
+     6121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
+     6181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacactaaat
+     6241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
+     6301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaaga
+//

wiki/pages/parts.html

@@ -72,6 +72,13 @@
           selectable marker for yeast and ampicillin resistance for bacterial
           selection, carried on a high-copy 2-micron vector.
         </p>
+        <p>
+          The original sequence of the pUDE1311 plasmid is available for
+          download
+          <a href="{{ url_for('static', filename='pUDE1311_sequenced.dna') }}"
+            >here</a
+          >.
+        </p>
         <div class="img-pagestyle">
           <img
             class="parts-img"
@@ -96,6 +103,23 @@
           />
           <figcaption>Figure 2: Constructs for yeast expression</figcaption>
         </div>
+        <p>
+          The sequences for the parts can be found in the Parts Registry.
+          Sequences for the parts inserted into the plasmid can be downloaded
+          here:
+          <a
+            href="{{ url_for('static', filename='pUDE1311_ymNeongreen_MTS1.dna') }}"
+            >MTS1</a
+          >,
+          <a
+            href="{{ url_for('static', filename='pUDE1311_ymNeongreen_uTP_1.dna') }}"
+            >uTP1</a
+          >,
+          <a
+            href="{{ url_for('static', filename='pUDE1311_ym_Neongreen_uTP_2.dna') }}"
+            >uTP2</a
+          >.
+        </p>
       </div>
 
       <div class="h" id="two">
@@ -144,6 +168,12 @@
           selectable marker (ble) containing an intron, and XbaI and KpnI
           restriction sites at the ends for cloning purposes.
         </p>
+        <p>
+          The original sequence of the pOpt2 plasmid is available for download
+          <a href="{{ url_for('static', filename='pOpt2_mVenus_Ble.gb') }}"
+            >here</a
+          >.
+        </p>
         <div class="img-pagestyle">
           <img
             class="parts-img"
@@ -168,6 +198,24 @@
             Figure 4: Constructs for <em>C. reinhardtii</em> expression
           </figcaption>
         </div>
+        <p>
+          The sequences for the parts can be found in the Parts Registry.
+          Sequences for the parts inserted into the plasmid can be downloaded
+          here:
+          <a href="{{ url_for('static', filename='pOPT2_mVenus_Ble_mTP.dna') }}"
+            >mtTP70C</a
+          >,
+          <a href="{{ url_for('static', filename='pOPT2_mVenus_Ble_cTP.dna') }}"
+            >CTP_PsaD</a
+          >,
+          <a
+            href="{{ url_for('static', filename='pOPT2_mVenus_Ble_uTP1.dna') }}"
+            >uTP1</a
+          >,
+          <a href="{{ url_for('static', filename='pOPT2_mVenus_Ble_uTP2.dna) }}"
+            >uTP2</a
+          >.
+        </p>
       </div>
 
       <div class="h" id="ref">