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Commit e8ac00dc authored by HouTeng Chan's avatar HouTeng Chan
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<h2 id="Dry Lab">
<h2>Dry Lab</h2>
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<p>In the dry lab, the prediction was made by <b>modeling</b>. Our model is divided into four interconnected parts, representing <b>the inhalation of muscone, its binding to receptors, intracellular signal transduction and lactic acid secretion triggered by receptor activation, and the absorption of lactic acid.</b> These models provide a comprehensive understanding of the project and yield valuable computational results. In addition, we have designed an <b>electronic sachets</b> with the implementation of therapy. We use emulsification via ultrasonication to make muscone evaporate from portable sachet bag and integrate traditional Chinese incense culture elements. For detailed information, please refer to <a href="https://2024.igem.wiki/Tsinghua/Model" style="color: #FF5151" >Model </a> or <a href="https://2024.igem.wiki/Tsinghua/Hardware" style="color: #FF5151" >Hardware </a> .</p>
<p>In the dry lab, the prediction was made by <b>modeling</b>. Our model is divided into four interconnected parts, representing <b>the inhalation of muscone, its binding to receptors, intracellular signal transduction and lactic acid secretion triggered by receptor activation, and the absorption of lactic acid.</b> These models provide a comprehensive understanding of the project and yield valuable computational results. In addition, we have designed an <b>electronic sachets</b> with the implementation of therapy. We use emulsification via ultrasonication to make muscone evaporate from portable sachet bag and integrate traditional Chinese incense culture elements. For detailed information, please refer to <a href="https://2024.igem.wiki/Tsinghua/model" style="color: #FF5151" >Model </a> or <a href="https://2024.igem.wiki/Tsinghua/hardware" style="color: #FF5151" >Hardware </a> .</p>
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<h2 id="Safety">
<h2>Safety</h2>
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<p>As a biological treatment we are developing, its safety has always been at the top priority for us. To ensure our engineered yeast can’t survive in the outside the gut, we introduced a suicide system using specific intestine marker, bile acid. In the intestine, bile acid binds to its receptor, which further activates the expression of CI protein. CI protein can inhibit the expression of suicide gene MazF. Once the yeast leaves intestine, lack of bile acid leads to successful expression of MazF, killing the engineered yeast. By introducing this system, we ensure that our yeast can only colonize and thrive in the intestine. Besides, nutritional-deficiency also provides a convenient and efficient approach for us to ensure biosafety. Our engineered yeast is uracil and histidine auxotrophic, further ensuring its safety. For detailed information, please refer to <a href="https://2024.igem.wiki/Tsinghua/Safety" style="color: #FF5151" >Safety </a>.</p>
<p>As a biological treatment we are developing, its safety has always been at the top priority for us. To ensure our engineered yeast can’t survive in the outside the gut, we introduced a suicide system using specific intestine marker, bile acid. In the intestine, bile acid binds to its receptor, which further activates the expression of CI protein. CI protein can inhibit the expression of suicide gene MazF. Once the yeast leaves intestine, lack of bile acid leads to successful expression of MazF, killing the engineered yeast. By introducing this system, we ensure that our yeast can only colonize and thrive in the intestine. Besides, nutritional-deficiency also provides a convenient and efficient approach for us to ensure biosafety. Our engineered yeast is uracil and histidine auxotrophic, further ensuring its safety. For detailed information, please refer to <a href="https://2024.igem.wiki/Tsinghua/safety" style="color: #FF5151" >Safety </a>.</p>
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