<figcaptionstyle="margin-top: -10px;">Figure9. The response of CueR-pCoA system upon
different Cu2+ concentrations over time.</figcaption>
</figure>
<br>
<p><strong>The development of a cell-free transcription test strip</strong></p>
<p>We first tested the cell lysis conditions using 75W 15min ultrasonic treatment for 2ml overnight culture. After plating the lysate, we observed that some bacterial cells were not fully lysed. In the second attempt, we increased the power to 90W, which successfully resulted in complete cell lysis as expected.</p>
<p>Next, we added Cu²⁺ to the lysate to test the system's response. However, a white precipitate formed in the solution, which we suspect was caused by the accidental addition of EDTA, leading to a reaction with Cu²⁺.</p>
<p>Due to time constraints and limited experimental conditions, we were unable to complete the functional validation of the system in the lysate. However, we have already designed the hardware for the entire system, aimed at future home-based detection applications. This setup will allow for easy and safe use outside of laboratory conditions, making it suitable for everyday use. The hardware is designed to integrate the cell-free system onto a user-friendly test strip, providing a reliable platform for real-time, at-home biosensing.</p>
