<p>Firstly, phenylethylamine (PEA) diffuses through the outer membrane of <em>Escherichia coli</em> into the periplasmic space, where it interacts with TynA.</p>
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<p>Based on the OptiGene algorithm, we identified two valuable target reactions: <b>Glycine Cleavage System (GLYCL)</b> and <b>Glutamate Dehydrogenase (NADP, GLUDy)</b>. The main reactions involved, the candidate knockout targets, and the effects of their knockout on flux are summarized in the table below:</p>