@@ -169,32 +169,32 @@ export function Experiments() {
<p>Content for section 2.</p>
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<h4className="center-text">LB medium preparation</h4>
<pclassName="indent">(1) Material
LB Broth Agar Powder, Miller
LB Broth Powder, Miller
Ultrapure (UP) Water
Double Distillation Water (ddH2O)
Ampicillin
Kanamycin
Chloramphenicol
(2) Steps
LB liquid medium
1. Weigh 12.5g of LB Broth Powder.
2. Add the powder into 500ml UP Water.
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
LB solid medium
1. Weigh 12g of LB Broth Agar Powder.
2. Add the powder into 300ml UP Water.
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
4. Pour the medium into culture dishes in the ultraclean worktable.
LB selective medium (Ampicillin)
1. Weigh 12g of LB Broth Agar Powder.
2. Add the powder into 300ml UP Water.
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
4. Weigh 1g of Ampicillin.
<pclassName="indent">(1) Material<br/>
LB Broth Agar Powder, Miller<br/>
LB Broth Powder, Miller<br/>
Ultrapure (UP) Water<br/>
Double Distillation Water (ddH2O)<br/>
Ampicillin<br/>
Kanamycin<br/>
Chloramphenicol<br/>
(2) Steps<br/>
LB liquid medium<br/>
1. Weigh 12.5g of LB Broth Powder.<br/>
2. Add the powder into 500ml UP Water.<br/>
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.<br/>
LB solid medium<br/>
1. Weigh 12g of LB Broth Agar Powder.<br/>
2. Add the powder into 300ml UP Water.<br/>
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.<br/>
4. Pour the medium into culture dishes in the ultraclean worktable.<br/>
LB selective medium (Ampicillin)<br/>
1. Weigh 12g of LB Broth Agar Powder.<br/>
2. Add the powder into 300ml UP Water.<br/>
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.<br/>
4. Weigh 1g of Ampicillin.<br/>
5. Add the ampicillin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully.
6. Filter the mixture through a filter to remove microorganism.
7. Add 500μL of mixture into an EP tube with 500μL ddH2O. The ddH2O is sterilized previously.
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@@ -205,17 +205,17 @@ LB selective medium (Kanamycin)
2. Add the powder into 300ml UP Water.
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
4. Weigh 100mg of Kanamycin.
5. Add the kanamycin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully.
6. Filter the mixture through a filter to remove microorganism.
7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.
LB selective medium (Chloramphenicol)
1. Weigh 12g of LB Broth Agar Powder.
2. Add the powder into 300ml UP Water.
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.
4. Weigh 500mg of Chloramphenicol.
5. Add the ampicillin into anhydrous ethanol and volume to 10mL. Shake the mixture fully.
6. Filter the mixture through a filter to remove microorganism.
5. Add the kanamycin into ddH2O and volume to 10mL. The ddH2O is sterilized previously. Shake the mixture fully.<br/>
6. Filter the mixture through a filter to remove microorganism.<br/>
7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.<br/>
LB selective medium (Chloramphenicol)<br/>
1. Weigh 12g of LB Broth Agar Powder.<br/>
2. Add the powder into 300ml UP Water.<br/>
3. Autoclave entire bottle of LB medium under 121°C for 20 minutes.<br/>
4. Weigh 500mg of Chloramphenicol.<br/>
5. Add the ampicillin into anhydrous ethanol and volume to 10mL. Shake the mixture fully.<br/>
6. Filter the mixture through a filter to remove microorganism.<br/>
7. When the LB medium cool down to nearly 50°C, add 300μL antibiotic mixture into it in the ultraclean worktable, then pour it into culture dishes.