@@ -253,9 +253,11 @@ Cycle 3: Validation of the regulation of the UV-responsive gene switch by RUP2 (
<p>In this cycle, we found that in the system without RUP2, co-transfection of all three plasmids greatly increased the expression of luciferase under UV induction. However, for the system containing RUP2, there was no significant difference in the luminescence intensity between co-transfection of all three plasmids and transfection of any two plasmids after UV induction. We speculate that this might be due to the high expression level of RUP2, which strongly inhibits the interaction between UVR8 and COP1, resulting in almost no efficient initiation of luciferase gene expression. Therefore, in the future, we will reduce the expression level and rate of RUP2 by increasing the number of rare codons on RUP2, so that the final expression level of the target gene stabilizes at an appropriate concentration.</p>
