<imgsrc="https://static.igem.wiki/teams/5342/images/experiments/procedure.webp"alt="Visual representation of design">
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<p>Created in BioRender.com</p>
<p>Figure 1. Overview of the process to reach the required protein Factor VIII.</p>
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The process timeline starts with transfecting pCDNA4 plasmids into E.coli bacteria strain where they are amplified. The linearization is carried out via XhoI restriction enzyme cleavage.
The pcDNA is transcribed into an mRNA sequence which undergoes 5’capping and 3’ poly-A tailing. The modified mRNA is encapsulated in our produced lipid nanoparticles.
These nanoparticles are introduced in HEK cell line where the the full FVIII protein is translated.