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Commit c4eac744 authored by Timofej Paramonov Bliki's avatar Timofej Paramonov Bliki :heart:
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Accidentally forgot the reason why I implemented manual classes for list...

Accidentally forgot the reason why I implemented manual classes for list showing and list hiding in my code. Updated measurement to fix the error and documented as a comment in all relevant files.
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......@@ -28,10 +28,7 @@
list-style: none;
font-size: 15pt;
}
.list-show li {
font-size: 15pt;
}
li {
.list-show li { /* Using li in style interferes with the header. */
font-size: 15pt;
}
.med-img {
......@@ -77,7 +74,7 @@
<h4>Prepare Nucleic Acid Solution
</h4>
<ul>
<ul class="list-show">
<li> Prepare 10 mL of 10 mM citrate buffer at pH 4.0. </li>
<li> Dissolve 3 mg of nucleic acid (e.g., mRNA) in 3 mL of the citrate buffer.</li>
</ul>
......@@ -94,7 +91,7 @@
</p>
<ul>
<ul class="list-show">
<li> Slowly inject the 1 mL ethanol-lipid solution into 3 mL of the citrate buffer containing the nucleic acid (1:3 volume ratio, which can be adjusted for the experiment's needs), while stirring the buffer rapidly.</li>
<li> The rapid mixing of the ethanol-lipid solution with the aqueous phase leads to the spontaneous formation of lipid nanoparticles.</li>
</ul>
......@@ -104,7 +101,7 @@
Equilibration:
</h5>
<ul>
<ul class="list-show">
<li> Continue stirring for 5-10 minutes to allow the system to equilibrate and ensure complete nanoparticle formation.</li>
</ul>
......@@ -112,7 +109,7 @@
Equilibration:
</h4>
<ul>
<ul class="list-show">
<li> Use a dialysis machine to remove the ethanol solvent (procedure description: [3], see video)</li>
</ul>
......@@ -120,7 +117,7 @@
Analysis
</h4>
<ul>
<ul class="list-show">
<li> Dynamic light scattering(DLS)</li>
<li> Encapsulation efficiency</li>
<li> Transmission Electron Microscopy (TEM)</li>
......@@ -130,7 +127,7 @@
Storage
</h4>
<ul>
<ul class="list-show">
<li> Without mRNA: RT</li>
<li> LNPs with mRNA store at: -80 C</li>
</ul>
......@@ -318,7 +315,7 @@
<h4>In our opinion, this constitutes the true value of measurement - not only the correct numbers, values, and units, but the useful conclusions and insights which are combined together to receive a solid background for future researchers.</h4>
<h2>Sources:</h2>
<ol>
<ol class="list-show">
<li> M. Kim et al. ,Engineered ionizable lipid nanoparticles for targeted delivery of RNA therapeutics into different types of cells in the liver.Sci. Adv.7,eabf4398(2021).DOI:10.1126/sciadv.abf4398 </li>
<li> Jung, Han Na, et al. “Lipid Nanoparticles for Delivery of RNA Therapeutics: Current Status and the Role of in Vivo Imaging.” Theranostics, vol. 12, no. 17, 2022, pp. 7509–7531, https://doi.org/10.7150/thno.77259. </li>
......
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