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Commit 49126a4d authored by Timofej Paramonov Bliki's avatar Timofej Paramonov Bliki :heart:
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Fixed image appearance in LNP

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......@@ -3117,10 +3117,12 @@
<ol>
<li>
10%MeOH/DCM: 0.5ml/4.5ml + triethyl amine: 0.2ml; Rf(1,2-epoxydodecane) = 0; Rf(1,4-Bis(3-aminopropyl) piperazine) = 1.5/6.3 = 0.24
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-3-webp.webp" alt="" class="journal-img">
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15%MeOH/DCM: 1.5ml/8.5ml + triethyl amine: 3 drops; Rf(1,2-epoxydodecane) = 0; Rf(1,4-Bis(3-aminopropyl) piperazine) 1/6.8= 0.15
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-4-webp.webp" alt="" class="journal-img">
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</ol>
......@@ -3130,24 +3132,29 @@
<ol>
<li>
15%MeOH/DCM: 2ml/11ml + triethyl amine: 5 drops (0.4 ml); Almost no starting material was seen. However, the Rf value was too big. Rf(product) = 4.7/5.1 = 0.92; Rf(1,4-Bis(3-aminopropyl) piperazine) = 0.5/4.4 = 0.113
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-5-webp.webp" alt="" class="journal-img">
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<li>
5%MeOH/DCM: 0.5ml/9.5ml + triethyl amine: 0.3 ml; Two spots were accidentally left for the product next to each other; Rf prod = 1.2 / 3.4 = 0.35; Rf epoxide = >0.95; Rf amine = 0.07;
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-6-webp.webp" alt="" class="journal-img">
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Reaction was left to run for 1 more day. TLC: 5%MeOH/DCM: 0.5ml/9.5ml + triethyl amine: 0.3 ml; TLC showed presence of product and epoxide and no signs of NH2, indicating that the reaction has gone to completion. RF product = 0.38; RF epoxide = 9.25; RF NH2 = 0.09
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-7-webp.webp" alt="" class="journal-img">
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</ol>
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The product was left in the freezer at -25C, as yellow crude oil.
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-8-webp.webp" alt="" class="journal-img">
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After 2 days, the product was taken out of the freezeer, it became yellowish solidified liquid. The weight of the unpurified compound was measured: 4.224 g.
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-9-webp.webp" alt="" class="journal-img">
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<li>The compound of the amount of: 1.018 g was run on the flash column.</li>
......@@ -3155,15 +3162,18 @@
<li>Eluent was prepared: 50 ml MeOH/ 950 ml DCM (5%MeOH/DCM), but triethylamine was not added.</li>
<li>
Fractions 4, 5, 14-26 were stained with annis stain. And fractions 4, 5, 6, 14 - 26 were stained with permanganate stain.
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-10-webp.webp" alt="" class="journal-img">
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TLC (5%MeOH/DCM + triethylamine 3 drops) has been done on the fractions: 3, 4, 5, 14, 18, 22, 26.
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-11-webp.webp" alt="" class="journal-img">
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<li>New fractions 1*-13* were made.</li>
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Fractions 14-3* showed the presence of compound, and were combined.
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<img src="https://static.igem.wiki/teams/5342/images/notebook/rad201-12-webp.webp" alt="" class="journal-img">
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<li>Fractions 14-3* were rotovapped 900-500 mbar, 55C, ~15min.</li>
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