<p>Designing DNA fragments for the Gibson assembly.</p>
<p><b>Nitrate Reduction Pathways</b></p>
<p>Nitrates can be removed from water through several bacterial metabolic processes. The most prevalent pathway is denitrification, in which NO₃⁻ is sequentially reduced to NO₂⁻ and then to N₂, which is released into the atmosphere (Zhao et al., 2018). Other important nitrate reduction pathways include dissimilatory NO₃⁻ reduction to NH₄⁺ (DNRA) and NO₃⁻ assimilation (Moreno-Vivián et al., 1999).</p>
