<p>Building the experimental setup for characterization.</p>
<p>This involves preparing cultures and conditions for testing.</p>
<p>This involves preparing cultures and conditions for testing.</p>
<p>This involves preparing cultures and conditions for testing.</p>
<p>With an outline of the theory behind the mechanism of the tests, the following plan was constructed.</p>
<p>A known concentration of engineered V. natriegens is to be placed in a solution that mimics the environmental conditions of the places where our GMO is meant to be implemented. This solution will contain a nitrate salt, to simulate the nitrate compounds from fertilisers leeched into the water. Nitrate sulfate would be optimal, due to the low interference of the sulfate ion on both the Griess test and the indophenol test. Additionally, it will contain some nourishment to allow the bacteria to survive for the time of the test.</p>
<p>Samples of the bacteria growing in the above described solution would then be collected at spaced increments of time, filtered from the bacteria via centrifugation and filtration, yielding a solution containing the compounds of interest (nitrites and ammonia).</p>
<p>The results of the test can be quantified by converting the absorbance of the sample, measured in a spectrophotometer against a blank, into concentration and compared to a calibration curve. The concentration the will be plotted over the time they were collected to establish the rate of conversion from nitrate to ammonia to finally determine the efficiency of the ANRA pathway of the newly engineered Vibrio natriegens.</p>
