As soon as our work in wet lab was done, we shared our results with clinicians inside and outside the province and received good feedback. Our probe provides deep tissue penetration and high resolution, enabling precise imaging in difficult-to-reach areas. In mouse models, this technique has successfully achieved precise imaging of the blood-testis barrier and the blood-brain barrier, and it shows great potential in other bioimaging applications, such as surgical navigation and quick intraoperative examination.