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2024 Competition
iZJU-China
Commits
988ae4a0
Commit
988ae4a0
authored
6 months ago
by
Shihong Yuan
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Update file engineering.html
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wiki/pages/engineering.html
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View file @
988ae4a0
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@@ -273,8 +273,8 @@ endblock %}
<p>
(1) To build the vector, we used BplⅠ and BamHⅠ to cut the vector and disrupt the TEF promoter, allowing us
to insert the CYC1 promoter and TSC10 into the plasmid.
</p>
<div
class=
"img-wrap"
>
<div
class=
"border"
style=
"
height: 150px;
display: flex;"
>
<img
src=
"https://static.igem.wiki/teams/5371/project/engineering/6.png"
style=
"object-fit: cover;"
>
<div
class=
"border"
style=
"display: flex;"
>
<img
src=
"https://static.igem.wiki/teams/5371/project/engineering/6
-1
.png"
style=
"object-fit: cover;"
>
</div>
<div
class=
"disc"
>
Figure 6 BplⅠ and BamHⅠare used to cut the vector, but did not delete the whole TEF promoter
</div>
</div>
...
...
@@ -315,8 +315,8 @@ endblock %}
<p>
(1) After consulting our instructors, we used NaeⅠ and BamHⅠ this time. Although NaeⅠ also cuts the f1 ori
region on the plasmid, it completely removes the TEF promoter.
</p>
<div
class=
"img-wrap"
>
<div
class=
"border"
style=
"
height: 150px;
display: flex;"
>
<img
src=
"https://static.igem.wiki/teams/5371/project/engineering/8.png"
style=
"object-fit: cover;"
>
<div
class=
"border"
style=
"display: flex;"
>
<img
src=
"https://static.igem.wiki/teams/5371/project/engineering/8
-1
.png"
style=
"object-fit: cover;"
>
</div>
<div
class=
"disc"
>
Figure 8 NaeⅠ and BamHⅠare used to cut the vector and deleted the whole TEF promoter
</div>
</div>
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