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Commit 7438fb34 authored by Siyuan Lyu's avatar Siyuan Lyu
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Update 2 files

- /wiki/pages/description.html
- /wiki/pages/engineering.html
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/description/fig1-pic2.jpg">
</div>
<div class="disc">Figure 1. The expanded market and custome's needs.</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/description/pic3.jpg">
</div>
<div class="disc">Figure 2. The ceramide synthesis pathway.</div>
</div>
<h2 id="2-2">&bull;&emsp;(-)-α-bisabolol production</h2>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/description/pic4.svg">
</div>
<div class="disc">Figure 3. The α-Bisabolol synthesis pathway.</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/description/pic5.svg">
</div>
<div class="disc">Figure 4. The microfluidic chip structure.</div>
</div>
</div>
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<p>(1) To build the vector, we used BplⅠ and BamHⅠ to cut the vector and disrupt the TEF promoter, allowing us
to insert the CYC1 promoter and TSC10 into the plasmid.</p>
<div class="img-wrap">
<div class="border" style="height: 150px;">
<img src="https://static.igem.wiki/teams/5371/project/engineering/6.png" style="object-fit: cover;transform: translateY(-150px);">
<div class="border" style="height: 150px;display: flex;">
<img src="https://static.igem.wiki/teams/5371/project/engineering/6.png" style="object-fit: cover;">
</div>
<div class="disc">Figure 6 BplⅠ and BamHⅠare used to cut the vector, but did not delete the whole TEF promoter</div>
</div>
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<p>(1) After consulting our instructors, we used NaeⅠ and BamHⅠ this time. Although NaeⅠ also cuts the f1 ori
region on the plasmid, it completely removes the TEF promoter.</p>
<div class="img-wrap">
<div class="border" style="height: 150px;">
<img src="https://static.igem.wiki/teams/5371/project/engineering/8.png" style="transform: translateY(-150px);">
<div class="border" style="height: 150px;display: flex;">
<img src="https://static.igem.wiki/teams/5371/project/engineering/8.png" style="object-fit: cover;">
</div>
<div class="disc">Figure 8 NaeⅠ and BamHⅠare used to cut the vector and deleted the whole TEF promoter</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/engineering/10.svg">
</div>
<div class="disc">Figure 10 The microfluidic chip structure.</div>
<div class="disc">Figure 10. The microfluidic chip structure.</div>
</div>
</div>
<h3>Build</h3>
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<div class="border" style="width: 35%;">
<img src="https://static.igem.wiki/teams/5371/project/engineering/11.svg">
</div>
<div class="disc">Figure 11 The microfluidic with tubes. Red box indicates the stress from the stainless steel tube.</div>
<div class="disc">Figure 11. The microfluidic with. Red box indicates the stress from the stainless steel tube.</div>
</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/engineering/12.png">
</div>
<div class="disc">Figure 12 The stainless steel was changed to the 90 degree bended stainless-steel tube.</div>
<div class="disc">Figure 12. The stainless steel was changed to the 90 degree bended stainless-steel tube.</div>
</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/engineering/13.svg">
</div>
<div class="disc">Figure 13 The 0.17mm coverslip enable us to test cell under the 60x microscope.</div>
<div class="disc">Figure 13. The 0.17mm coverslip enable us to test cell under the 60x microscope.</div>
</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/engineering/14.svg">
</div>
<div class="disc">Figure 14 The jig of the chip.</div>
<div class="disc">Figure 14. The jig of the chip.</div>
</div>
</div>
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<div class="border">
<img src="https://static.igem.wiki/teams/5371/project/engineering/11.jpg">
</div>
<div class="disc">Figure 15 The focus shift was largely reduced by using the chip jig.</div>
<div class="disc">Figure 15. The focus shift was largely reduced by using the chip jig.</div>
</div>
</div>
</div>
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