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Commit 5f85d39d authored by Avani Sardana's avatar Avani Sardana
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......@@ -8,11 +8,4 @@ To take a break from the stress of research work and academics, iGEM IISc organi
Participants were divided into four teams, and took turns picking chits from a box, which listed words related to Synthetic Biology, and drew them for their team to guess. The experience was a fun one, and even presented the opportunity to learn new things in the process. All the participants had an evening full of laughs, a welcome break before they went back to their tight schedules.
<div class="row padding">
<div class="col-6">
<img src="https://static.igem.wiki/teams/5170/hp-images/pictionary1.jpeg" alt="Pictionary">
</div>
<div class="col-6">
<img src="https://static.igem.wiki/teams/5170/hp-images/pictionary2.jpeg" alt="Pictionary">
</div>
</div>
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<img src="https://static.igem.wiki/teams/5170/hp-images/pictionary2.jpeg"
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......@@ -5,4 +5,6 @@ author: Suvam Saswat Das
While sifting through different protocols and newer systems of SELEX, we chanced upon the work of an iGEM team, OLM Madrid, that had tried to develop a more efficient system of SELEX. We consulted **Laura Armero Hernandez** from their team.
The meeting (facilitated via an online platform) provided many insights about SELEX, how tedious it can be, and the effectiveness of the result it provides. The method they used also took around 3-4 months to produce an effective and more selective aptamer. Considering the time constraint and our plan of action, Laura suggested that we avoid performing SELEX (since it was not the central feature of our idea but only a part of it). She instead encouraged us to use pseudo-SELEX or directly use an established aptamer.
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The meeting (facilitated via an online platform) provided many insights about SELEX, how tedious it can be, and the effectiveness of the result it provides. The method they used also took around 3-4 months to produce an effective and more selective aptamer. Considering the time constraint and our plan of action, Laura suggested that we avoid performing SELEX (since it was not the central feature of our idea but only a part of it). She instead encouraged us to use pseudo-SELEX or directly use an established aptamer.
<img src = "https://static.igem.wiki/teams/5170/hp-images/madrid.jpeg", style = "max-height: 300px; max-widht: auto; display: block; margin-left: auto; margin-right: auto;}" alt = "gene">
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......@@ -7,6 +7,10 @@ We had series of discussions and deliberations with Prof. Nair to understand how
Dr. Nagendran Ramalingam was so excited upon hearing the idea that he invited his colleagues **Prof. Jean-Pierre Bellier** and **Prof. Lei Liu,** of Brigham and Women's Hospital, Harvard Medical School. They too shared much enthusiasm about the idea and were positive about its outcomes. However, they were sceptical about certain points and raised their concerns about the difficulties they believed we would face.
<img src = "https://static.igem.wiki/teams/5170/hp-images/liu.jpeg", style = "max-height: 300px; max-widht: auto; display: block; margin-left: auto; margin-right: auto;}" alt = "gene">
The idea of phosphorylation, though could be used, was to be carefully planned and executed. Prof. Liu had the same opinion as most others we consulted about the process of SELEX, given the time constraint. From the meeting we received valuable inputs regarding phosphorylation, cell health assay and pseudo-SELEX process among others.
Halfway through our work, we had another meeting to update Dr. Ramalingam and his colleagues about the progress and received feedback for the same. They did help us plan our work efficiently, and also provided us with suggestions on testing and finalization of our aptamer.
<img src = "https://static.igem.wiki/teams/5170/hp-images/liu-2.jpeg", style = "max-height: 300px; max-widht: auto; display: block; margin-left: auto; margin-right: auto;}" alt = "gene">
......@@ -3,4 +3,6 @@ title: Meeting with Students from IIT Delhi
author: Suvam Saswat Das
---
Running parallel to the meetings and discussions about work in the wet lab, we were also making considerably progress in planning dry lab activities. We had a meeting with **ex-iGEMers from IIT Delhi,** who had developed pipelines and console for simulation and predictors. Since the two projects did not align much, we could not collaborate with them further. They, however, did provide valuable information and suggestions related to dry lab work, such as protein simulation and molecular modelling, among others, during the meeting.
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Running parallel to the meetings and discussions about work in the wet lab, we were also making considerably progress in planning dry lab activities. We had a meeting with **ex-iGEMers from IIT Delhi,** who had developed pipelines and console for simulation and predictors. Since the two projects did not align much, we could not collaborate with them further. They, however, did provide valuable information and suggestions related to dry lab work, such as protein simulation and molecular modelling, among others, during the meeting.
<img src = "https://static.igem.wiki/teams/5170/hp-images/iitd.jpeg", style = "max-height: 300px; max-widht: auto; display: block; margin-left: auto; margin-right: auto;}" alt = "gene">
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......@@ -3,4 +3,7 @@ title: iGEM Mentor- Dr. Roghaiyeh Safari
author: Suvam Saswat Das
---
We also had meetings with our iGEM mentor, Dr. Roghaiyeh Safari, Imperial College, London. She enquired about our business models, along with our idea and implementation. Her valuable feedback on business models and our pitch deck was utilised in our design. She did provoke our thoughts about the market and entrepreneurship. She also helped review our pitch decks and plan of action.
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We also had meetings with our iGEM mentor, Dr. Roghaiyeh Safari, Imperial College, London. She enquired about our business models, along with our idea and implementation. Her valuable feedback on business models and our pitch deck was utilised in our design. She did provoke our thoughts about the market and
entrepreneurship. She also helped review our pitch decks and plan of action.
<img src = "https://static.igem.wiki/teams/5170/hp-images/rugi.jpeg", style = "max-height: 300px; max-widht: auto; display: block; margin-left: auto; margin-right: auto;}" alt = "gene">
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......@@ -100,7 +100,11 @@
was one single team, unified with science, bonded with dreams.
</p>
<img align="center" src="https://static.igem.wiki/teams/5170/hp-images/ideareveal.png" alt="" style="height: 400px; width: 100%; object-fit: cover; overflow:hidden;">
<br>
<p class="padding">
<br>
The team had to get the coordinators and leader and that’s why the experience in the cycle wasn’t only
of a scientific rollercoaster. The team voted me as the Team Leader and Suvam as the co-Team Leader.
From administering a team to facing the issues with the administration, it was all like the lines of a
......@@ -128,6 +132,9 @@
Manya the reserved lady, Avani the bold girl, Arjun the innocent, Anushka the illustrator, Geeth with the
distinctive accent, Harish who laughs more than he speaks, Shouvik the Neuroscientist and many more…
</p>
<img src="https://static.igem.wiki/teams/5170/hp-images/ice-cream.jpeg" alt="" style="height: 600px; width: 80%; object-fit: cover; overflow:hidden;">
<hr>
<div style="height: 5rem;"></div>
</div></div>
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......@@ -8,6 +8,7 @@
<h1 class="text-center padding display-4"><b>Blog Posts</b></h1>
<div class="d-flex flex-wrap row">
{% for blog in bloglist: %}
<div class="card-mb-4 col-lg-3 col-md-6 col-sm-12">
<div class="card-body">
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......@@ -7,6 +7,10 @@
<link href="{{ url_for('static', filename = 'edublog.css') }}" rel="stylesheet">
<h1 class="text-center padding display-4"><b>Education</b></h1>
<p>
Through Education, we aimed to spread awareness about STEM and Synthetic Biology, as well as educate the general public about our ongoing work. Apart from the blogposts on our wiki, our work has been documented below.
</p>
<div class="d-flex flex-wrap row">
{% for blog in edubloglist: %}
<div class="card-mb-4 col-lg-3 col-md-6 col-sm-12">
......
......@@ -7,6 +7,10 @@
<link href="{{ url_for('static', filename = 'blog.css') }}" rel="stylesheet">
<h1 class="text-center padding display-4"><b>Integrated Human Practices</b></h1>
<p>
Our Integrated Human Practices involved speaking to stakeholders such as doctors, nurses, caretakers, friends and family of patients, as well as those carrying out ongoing research in the field. Below, we have documented our journey throughout the project, from the genesis of the idea, through all the failures, rejections and suggestions and modifications - because of which it is as it is today. We have also demonstrated how we incorporated the feedback we received at each step along the way. We are grateful to all our mentors and our advisors who have helped us along the way.
</p>
<div class="d-flex flex-wrap row">
{% for blog in ihpbloglist: %}
<div class="card-mb-4 col-lg-3 col-md-6 col-sm-12">
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......@@ -37,7 +37,7 @@
</div> -->
<h1 class="padding text-center">Lab Notebook</h1>
<div style="text-align: center;">
<button class="toggle-btn" onclick="showPDF('https://static.igem.wiki/teams/5170/protein-expression.pdf')">Protein Expression</button>
<button class="toggle-btn" onclick="showPDF('https://static.igem.wiki/teams/5170/comp-cell-and-prot-exp.pdf')">Protein Expression</button>
<button class="toggle-btn" onclick="showPDF('https://static.igem.wiki/teams/5170/protein-purification-and-verification.pdf')">Protein Purification and Verification</button>
<button class="toggle-btn" onclick="showPDF('https://static.igem.wiki/teams/5170/phosphorylation.pdf')">Phosphorylation</button>
<button class="toggle-btn" onclick="showPDF('https://static.igem.wiki/teams/5170/clickfinal.pdf')">Click Reaction and binding of Aptamers</button>
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......@@ -17,9 +17,18 @@
</head>
<body>
<h1 style="text-align: center;">Lab Results</h1>
<iframe src="https://static.igem.wiki/teams/5170/results.pdf"></iframe>
<h1 style="text-align: center;">Lab Results</h1>
<iframe src="https://static.igem.wiki/teams/5170/results.pdf"></iframe>
<h3 class="mt-3 text-center padding">
Further Plans
</h3>
<p>
Once our aptamers our generated, along with our work with Targeted Protein Delivery, we wish to parallely check out some ideas.
</p>
<p>
Diagnosis: By conjugating our aptamer with a light sensitive molecule, we hope to achieve for a tool which can help in diagnosis. Since reports have shown that p231 Tau can play a very important role in preclinical diagnosis of Alzheimer's, we would hope to develop this into an assay that can streamline the development of further diagnosis tools on CSF/plasma samples. This will help in a better understanding of the disease in a person in conjunction with other tests.
</p>
{% endblock %}
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