Following the successful transformation of the pET28-c_resilin plasmid into competent JM109 bacteria of the novel reCA-deficient expression strain, the expression of resilin was induced with IPTG. After 21 hours, the cells were either lysed with only SDS-sample buffer (Figure 11), sonification (Figure 11) or heat (Figure 12), and subsequently analysed by SDS-PAGE.
Following the successful transformation of the pET28-c_resilin plasmid into competent JM109 bacteria of the novel reCA-deficient expression strain, the expression of resilin was induced with IPTG. After 21 hours, the cells were either lysed with only SDS-sample buffer (Figure 11), sonification (Figure 12) or heat (Figure 13), and subsequently analysed by SDS-PAGE.