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Commit dbcee986 authored by Qianyue Jin's avatar Qianyue Jin
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Update file safety.html

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<div class="inspirationtext">
Dienamite is composed of three primary components: spider venom peptides (SVPs), zingiberene, and harpin protein.<br><br> During our initial research, aiming to exterminate red spider mites, SVP, a type of venom peptide particularly
effective at disrupting neuronal receptors and voltage-gated ion channels, caught our attention. With high specificity, SVPs are highly toxic to spider mites, and are non-toxic to mammals and pollinators. Meanwhile, we surprisingly
discovered that the teams NCTU_Formosa(<a href=" https://2016.igem.org/Team:NCTU_Formosa"> https://2016.igem.org/Team:NCTU_Formosa</a>) and UAM(<a href="https://2022.igem.wiki/uam/index.html">https://2022.igem.wiki/uam/index.html</a>)also
discovered that the teams NCTU_Formosa(<a href=" https://2016.igem.org/Team:NCTU_Formosa"> https://2016.igem.org/Team:NCTU_Formosa</a>) and UAM (<a href="https://2022.igem.wiki/uam/index.html">https://2022.igem.wiki/uam/index.html</a>)also
incorporated SVPs in their project.<br><br>We also conducted gene alignment for the genome of P. Persimilis, a predatory mite with widespread commercial use that consumes only spider mites, to obtain more specialized venom peptides
, thus gaining a novel venom peptide MVP. MVPs are even more specific to spider mites and pose a reduced risk to non-target organisms. After confirming the specificity and thus safety of the SVP and MVP, we designed our DNA sequence
and synthesized them through gene synthesis companies, after which we assembled them and transform them into selected chassis.<br><br>After our check-in form was approved by iGEM, we sent our successfully expressed SVPs and MVPs to
Professor Huang of SCAU. He followed the principles of testing according to the Guideline for laboratory bioassay of pesticides provided by NY/T 1154.13-2008 and tested the contact toxicity of our SVPs and MVPs on female Tetranychus
urticae, a representative of spider mites.<br><br>Meanwhile, we also fully investigated the processes and requirements for pesticide registration: toxicological, environmental, pharmacodynamic and pesticide residue evaluations must
Professor Huang of SCAU. He followed the principles of testing according to the Guideline for laboratory bioassay of pesticides provided by NY/T 1154.13-2008 and tested the contact toxicity of our SVPs and MVPs on female <i>Tetranychus
urticae</i>, a representative of spider mites.<br><br>Meanwhile, we also fully investigated the processes and requirements for pesticide registration: toxicological, environmental, pharmacodynamic and pesticide residue evaluations must
be carried out before our product can be officially sold on the market. With great hopes of launching our product on the market, we will test the toxicity and decomposition of our venom peptides according to the regulations of the
government.<br><br>
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