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Commit 16c10281 authored by Qianyue Jin's avatar Qianyue Jin
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thaliana</i> respectively, 9HZ and 9H10epoZ are produced from further oxidation of 7epiZ [20]. To imitate anchorage to ER of the reductase and oxidase in plants, that brought about the two enzymes to proximity to allows efficient
electron transfer, we opted to use SpyTag-SpyCatcher system[Fig.10], acting as a flexible bridge linking the oxidase and the reductase [21]. This ensures efficient electron transfer and thus efficient biosynthesis.<br><br>
<figure style="text-align: center;"><img src="https://static.igem.wiki/teams/5184/description/zingiberene-pathway2.svg" style="width: 30vw;display: inline-block;">
<figcaption class="figure-note"><strong>Fig.9</strong>| Pathway diagram for zingiberene and subsquent oxidations that give 9H10epoZ with glucose as the initial carbon source (glycolysis and synthesis of acetoacetyl-CoA from acetyl-CoA is omitted in the diagram)</figcaption>
<figcaption class="figure-note"><strong>Fig.9</strong>| Pathway diagram for zingiberene and subsquent oxidations that give 9H10epoZ from any source of acetyl-CoA</figcaption>
</figure><br>
<figure style="text-align: center;"><img src="https://static.igem.wiki/teams/5184/description/cyp-cpr-spytag-spycatcher2.svg" style="width: 45vw;display: inline-block;">
<figcaption class="figure-note"><strong>Fig.10</strong>| SpyTag/SpyCatcher system allows attachment of SlCPR2 to ShZPO. This facilitates electron transfer between the two enzymes and thus allows more efficient catalysis by ShZPO that transforms 7epiZ to 9HZ and
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