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Commit 6224919f authored by Vishwaa Kannan's avatar Vishwaa Kannan
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<a class="nav-link" href="#design">Parts Design</a>
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<a class="nav-link" href="#kms">References</a>
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<a class="nav-link" href="#modelling">Modelling</a>
<a class="nav-link" href="#modeling">Modelling</a>
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<a class="nav-link" href="#vcell">Vcell</a>
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<p>
To test our constructs, we would transform them into E. coli and incubate them in varying levels of PFOA
(perfluorooctanoic acid) in 96-well plates. Fluorescence readings were taken of each well at 90 minute time
intervals and recorded. More information can be seen on the Experiments and Results page. Each gene insert was
flanked with BsaI and SapI sites and then printed from Genscript in pUC57 family carrier plasmids.
intervals and recorded. More information can be seen on the Experiments and Results page. Each gene insert was
flanked with BsaI and SapI sites and then printed from Genscript in pUC57 family carrier plasmids.
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<p>
↑ Mann, M. M., & Berger, B. W. (2023, September 13). A genetically-encoded biosensor for direct
detection of perfluorooctanoic acid. Nature News. https://www.nature.com/articles/s41598-023-41953-1
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↑ Smathers, R. L., & Petersen, D. R. (2011, March 1). The human fatty acid-binding protein family:
Evolutionary divergences and functions - human genomics. BioMed Central.
https://humgenomics.biomedcentral.com/articles/10.1186/1479-7364-5-3-170
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↑ Tuttle, A. R., Trahan, N. D., & Son, M. S. (2021). Growth and maintenance of escherichia coli
laboratory strains. Current Protocols, 1(1). https://doi.org/10.1002/cpz1.20
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<p>
↑ Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A. Growth phase-dependent variation in protein
composition of the Escherichia coli nucleoid. J Bacteriol. 1999 Oct;181(20):6361-70. doi:
10.1128/JB.181.20.6361-6370.1999. PMID: 10515926; PMCID: PMC103771.
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<p>
Dossani, Z. Y., Reider Apel, A., Szmidt-Middleton, H., Hillson, N. J., Deutsch, S., Keasling, J. D., &
Mukhopadhyay, A. (2018, March). A combinatorial approach to synthetic transcription factor-promoter
combinations for yeast strain engineering. Yeast (Chichester, England).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873372/
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ACS Publications: Chemistry journals, books, and references published ... (n.d.).
http://pubs.acs.org/doi/full/10.1021/ja026939x?mobileUi=0
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<h2>Modeling</h2>
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energy calculations, providing insights into how changes (like mutations in a protein) might affect binding
strength. In simple terms, it’s a way to measure how well two molecules stick together, which is crucial for
understanding processes like drug efficacy or protein interactions.</p>
<p>In order to carry out these processes, we used AutDock Vina and Amber</p>
<p>In order to carry out these processes, we used AutoDock Vina and Amber</p>
<h4>References</h4>
<ul>
<li>Eberhardt, J., Santos-Martins, D., Tillack, A.F., Forli, S. (2021). AutoDock Vina 1.2.0: New Docking
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<div id="human">
<h2>Human Practices</h2>
<hr>
<p>Our human practices focus on how innovative detection methods can benefit communities. We engaged with a variety of stakeholders, including researchers, environmental advocates, local citizens, and went on water company tours to ensure that our project aligns with public health needs and ethical standards. By prioritizing transparency and community input, we want to address concerns about health risks posed by PFAS, especially in underrepresented and vulnerable populations.
<p>Our human practices focus on how innovative detection methods can benefit communities. We engaged with a
variety of stakeholders, including researchers, environmental advocates, local citizens, and went on water
company tours to ensure that our project aligns with public health needs and ethical standards. By
prioritizing transparency and community input, we want to address concerns about health risks posed by PFAS,
especially in underrepresented and vulnerable populations.
</p>
<p>We are also committed to educational outreach, raising awareness about PFAS contamination and the potential impact of detection technologies. By collaborating with environmental organizations and policy makers, we aim to provide the public with the knowledge and tools they need to advocate for cleaner environments. Through human practices, we hope to solidify the relationship between the scientific community and the public, ensuring that our innovations serve beneficially to change.
<p>We are also committed to educational outreach, raising awareness about PFAS contamination and the potential
impact of detection technologies. By collaborating with environmental organizations and policy makers, we aim
to provide the public with the knowledge and tools they need to advocate for cleaner environments. Through
human practices, we hope to solidify the relationship between the scientific community and the public,
ensuring that our innovations serve beneficially to change.
</p>
</div>
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