@@ -326,10 +326,11 @@ function AnalyseWischmeyer(){
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}
functionAnalyseMattijs(){
const{goToPagesAndOpenTab}=useNavigation();
return(
<Collapsibletitle="Mattjis Bulcaen – University of Leuven, CF and Prime Editing Expert"id="mattijsanalyseC">
<p>Mattijs Bulcaen, being a researcher working on a topic very close to ours, provided invaluable guidance in the early stages of our project. He gave us an insight into the current advances in the field that we that we were able to make use of later on. He reviewed with us our own ideas and considerations, such as the use of the PEAR reporter system. Following our interview with Mattijs we integrated the structural motif trevopreQ1 into the pegRNA, which enhanced the prime editing efficiency - a critical improvement we successfully tested and demonstrated in our results. </p>
<p>Our decision of first testing prime editing in HEK293 cells instead of other cell lines or primary cells was also based on his statement that HEK cells are by far the easiest to archieve editing in. Mattijs additionally recommended the use of HEK293T cell lines overexpressing CFTR and CFTR F508del[link]. However, after conducting patch clamp measurements, we found that the HEK cells were unsuitable for our needs, requiring us to adjust our testing system. As a result, we switched to Ignatova[Link] cells from Hamburg for further experiments. </p>
<p>Our decision of first testing prime editing in HEK293 cells instead of other cell lines or primary cells was also based on his statement that HEK cells are by far the easiest to archieve editing in. Mattijs additionally recommended the use of HEK293T cell lines overexpressing CFTR and CFTR F508del[link]. However, after conducting patch clamp measurements, we found that the HEK cells were unsuitable for our needs, requiring us to adjust our testing system. As a result, we switched to <aonClick={()=>goToPagesAndOpenTab('ignatova','/human-practices')}>Ignatova's</a> cells from Hamburg for further experiments. </p>