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Commit dd76a1b3 authored by Kaya Lange's avatar Kaya Lange
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Merge branch 'main' of ssh://gitlab.igem.org/2024/bielefeld-cebitec

parents 87b2a512 0b065bcb
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src/.DS_Store
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src/App/mediarules.css
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......@@ -113,9 +113,8 @@
/***Biosafty***/
#safehead {
background-size: contain; /* Adjust the image to fit within the container */
background-position: center; /* Push the image down for better visibility */
background-position: center 35%; /* Push the image down for better visibility */
height: 300px; /* Adjust the height for mobile screens */
padding-top: 0px; /* Add padding to push down content and make image more visible */
}
/***Description***/
#Cystic\ Fibrosis6 h2 {
......
src/components/Tabs.tsx
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− 13
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......@@ -37,10 +37,13 @@ import { stringToSlug } from "../utils";
else if(data[i].language === "de"){
lang = "Original language: German"
}
else if(data[i].type != "meta"){
else{
lang = ""
}
/* else if(data[i].type != "meta"){
problem = true;
problem_desc.push("interview language");
}
} */
/* Expert on */
let expert = "";
......@@ -71,7 +74,27 @@ import { stringToSlug } from "../utils";
problem = true;
problem_desc.push("quote missing");
} */
let inv_img: React.ReactNode = <></>;
let imp_img: React.ReactNode = <></>;
let aim_img: React.ReactNode = <></>;
// Restliche Logik der Schleife...
// Bildzuweisungen basierend auf den Daten
if (data[i].pictureurl_interview) {
inv_img = <div className="hti-box">
<img src={data[i].pictureurl_interview} className="hp-timeline-img hti-right" />
</div>;
}
if (data[i].pictureurl_implementation) {
imp_img = <div className="hti-box">
<img src={data[i].pictureurl_implementation} className="hp-timeline-img hti-right" />
</div>;
}
if (data[i].pictureurl_aim) {
aim_img = <div className="hti-box">
<img src={data[i].pictureurl_aim} className="hp-timeline-img hti-left" />
</div>;
}
/* textparts */
let aiM: JSX.Element = <></>
if(data[i].aimofcontact){
......@@ -141,7 +164,7 @@ import { stringToSlug } from "../utils";
problem = true;
problem_desc.push(" cardtext missing");
} */
if(data[i].type !== "meta"){
/* if(data[i].type !== "meta"){
if(data[i].affiliation === undefined || data[i].affiliation === ""){
problem = true;
problem_desc.push(" affiliation missing");
......@@ -150,7 +173,7 @@ import { stringToSlug } from "../utils";
problem = true;
problem_desc.push(" job missing" );
}
}
} */
/* if(data[i].type === "meta"){
if(data[i].quoteVorname === undefined || data[i].quoteVorname === ""){
problem = true;
......@@ -165,27 +188,41 @@ import { stringToSlug } from "../utils";
}
/* Further pictures */
var inv_img: React.ReactNode = <></>;
/* var inv_img: React.ReactNode = <></>;
if (data[i].pictureurl_interview) {
inv_img = <> <div className="hti-box"> <img src={data[i].pictureurl_interview} className="hp-timeline-img hti-right"/> </div> <div className="hti-abstand"/> </>
console.info("before: inv_img is: " + inv_img)
console.info(data[i].vorname + " has pictureurl_interview")
inv_img = <> <div className="hti-box"> <img id={`${data[i].vorname}-inv-img`} src={data[i].pictureurl_interview} className="hp-timeline-img hti-right"/> </div> <div className="hti-abstand"/> </>
console.info("after: inv_img is: " + inv_img)
}
var imp_img: React.ReactNode = <></>;
if (data[i].pictureurl_implementation) {
imp_img = <> <div className="hti-abstand"/><div className="hti-box">
<img src={data[i].pictureurl_implementation} className="hp-timeline-img hti-right"/>
console.info(data[i].vorname + " has pictureurl_implementation")
console.info("before: imp_img is: " )
console.info(imp_img)
imp_img = <> <div className="hti-abstand"/><div className="hti-box">
<img id={`${data[i].vorname}-imp-img`} src={data[i].pictureurl_implementation} className="hp-timeline-img hti-right"/>
</div>
</>
console.info("after: imp_img is: " )
console.info(imp_img)
}
var aim_img: React.ReactNode = <></>;
if (data[i].pictureurl_aim) {
console.info(data[i].vorname + " has pictureurl_aim")
console.info("before: aim_img is: " )
console.info(aim_img)
aim_img = <> <div className="hti-abstand"/> <div className="hti-box">
<img src={data[i].pictureurl_aim} className="hp-timeline-img hti-left"/>
</div></>
}
<img id={`${data[i].vorname}-aim-img`} src={data[i].pictureurl_aim} className="hp-timeline-img hti-left"/>
</div></>
console.info("after: aim_img is: " )
console.info(aim_img)
} */
let int = <></>;
let int_id = stringToSlug(`${data[i].vorname}-inv-collapsible`)
if (data[i].interview) {
int = <Collapsible title="Interview" id={int_id}>
{data[i].interview}
</Collapsible>
......@@ -205,6 +242,9 @@ import { stringToSlug } from "../utils";
}
let newlist = oldpics.concat(data[i].more_pictures!);
for (let ind = 0; ind < (newlist.length - 1); ind += 2) {
console.info(data[i].vorname)
console.info(ind + newlist[ind])
console.info(ind+1 + newlist[ind+1])
morepics.push(
<div className="row">
<div className="col">
......
src/contents/.DS_Store
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src/contents/Human Practices/Conclisuin.tsx
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......@@ -21,7 +21,7 @@ export function HPconclusion(){
<H5 text="Feedback Loops and Project Evolution "/>
<p>We employed a structured feedback cycle based on Gibbs' Reflection Cycle, ensuring our design continuously evolved with stakeholder input. From initial design to public outreach, every phase of PreCyse was shaped by the feedback we received, allowing us to develop a patient-centered and globally relevant solution. </p>
<H5 text="Documentation and Future Guidance "/>
<p>We are committed to transparency and open science. Our detailed documentation of stakeholder interactions and methods—including protocols for working with human nasal epithelial cells [Link]—provides a foundation for future iGEM teams. Our outreach efforts, including educational materials and public engagement campaigns, help foster a broader understanding of CF and gene therapy, reflecting iGEM’s vision of responsible and impactful scientific practices. </p>
<p>We are committed to transparency and open science. Our detailed documentation of stakeholder interactions and methods—including protocols for working with <a onClick={() => goToPageAndScroll ('Cell Culture3', '/materials-methods')}> human nasal epithelial cells </a>—provides a foundation for future iGEM teams. Our outreach efforts, including educational materials and public engagement campaigns, help foster a broader understanding of CF and gene therapy, reflecting iGEM’s vision of responsible and impactful scientific practices. </p>
<H4 text="Next Steps"/>
<ol>
......@@ -327,10 +327,11 @@ function AnalyseWischmeyer(){
}
function AnalyseMattijs(){
const {goToPagesAndOpenTab} = useNavigation();
const {goToPageAndScroll} = useNavigation();
return(
<Collapsible title="Mattjis Bulcaen – University of Leuven, CF and Prime Editing Expert" id="mattijsanalyseC">
<p>Mattijs Bulcaen, being a researcher working on a topic very close to ours, provided invaluable guidance in the early stages of our project. He gave us an insight into the current advances in the field that we that we were able to make use of later on. He reviewed with us our own ideas and considerations, such as the use of the PEAR reporter system. Following our interview with Mattijs we integrated the structural motif trevopreQ1 into the pegRNA, which enhanced the prime editing efficiency - a critical improvement we successfully tested and demonstrated in our results. </p>
<p>Our decision of first testing prime editing in HEK293 cells instead of other cell lines or primary cells was also based on his statement that HEK cells are by far the easiest to archieve editing in. Mattijs additionally recommended the use of HEK293T cell lines overexpressing CFTR and CFTR F508del[link]. However, after conducting patch clamp measurements, we found that the HEK cells were unsuitable for our needs, requiring us to adjust our testing system. As a result, we switched to <a onClick={() => goToPagesAndOpenTab('ignatova', '/human-practices')}>Ignatova's</a> cells from Hamburg for further experiments. </p>
<p>Our decision of first testing prime editing in HEK293 cells instead of other cell lines or primary cells was also based on his statement that HEK cells are by far the easiest to archieve editing in. Mattijs additionally recommended the use of <a onClick={() => goToPageAndScroll ('Cell Culture', '/materials-methods')}> HEK293T cell lines </a> overexpressing CFTR and CFTR F508del. However, after conducting patch clamp measurements, we found that the HEK cells were unsuitable for our needs, requiring us to adjust our testing system. As a result, we switched to <a onClick={() => goToPagesAndOpenTab('ignatova', '/human-practices')}>Ignatova's</a> cells from Hamburg for further experiments. </p>
<figure>
<div className="row">
<div className="col">
......@@ -562,7 +563,7 @@ function AnalyseRnhale(){
<p>Dr. Benjamin Winkeljann from <a href="https://rnhale.com/" >Rnhale</a> provided crucial technical guidance that significantly enhanced our project. His expertise in spray-dried lipid nanoparticles (LNPs) allowed us to improve the shelf-life and scalability of our formulations. We learned about the limitations of CF therapies regarding international access, cost, and availability, which highlighted the need for sustainable solutions. </p>
<p>We discovered that spray drying is an effective method for stabilizing LNPs, enabling transport without refrigeration — a more environmentally friendly approach that reduces energy consumption. </p>
<p>Our close collaboration with RNhale will continue even after the wiki freeze, focusing on producing spray-dried LNPs and testing them on our primary cultures. We are also investigating how to enhance the stability of LNPs, particularly in protecting RNA from heat damage, and are seeking experts in chitosan for further support. </p>
<p>During discussions with a young startup entrepreneur, we explored the possibility of pursuing an <a onClick={() => goToPageAndScroll ('Further Engagement3H', '/human-practice')}> entrepeneuship-oriented </a> project but ultimately decided to focus on a human-centered approach that prioritizes the needs of CF patients over cost-driven industrial pathways. </p>
<p>During discussions with a young startup entrepreneur, we explored the possibility of pursuing an <a onClick={() => goToPageAndScroll ('Further Engagement3H', '/human-practices')}> entrepeneuship-oriented </a> project but ultimately decided to focus on a human-centered approach that prioritizes the needs of CF patients over cost-driven industrial pathways. </p>
<figure>
<div className="row">
<div className="col">
......@@ -588,10 +589,12 @@ function AnalyseRnhale(){
)
}
function AnalyseJoshua(){
const {goToPagesAndOpenTab} = useNavigation();
const {goToPageAndScroll} = useNavigation();
return(
<Collapsible title="Joshua – Vice president of CF Vest international, Father of a CF child" id="joshuaanalyseC">
<p>Through discussions with Joshua[Link] [HP_Interview_Joshua.docx ], we learned that CF statistics are inadequate, primarily representing the white population, which skews understanding of the disease's prevalence. We discovered that in Asian countries like Thailand, CF is underrepresented, leading to insufficient access to therapies and medications. </p>
<p>This awareness sharpened our focus on the need for improved science communication and highlighted the ongoing issues of racism and discrimination within scientific research. In response, we are committed to enhancing the data landscape in Germany by creating surveys[Link] [PO_Survey Public and Patients.docx ] in both German and English to gather broader insights and increase outreach. </p>
<p>Through discussions with <a onClick={() => goToPagesAndOpenTab ('joshua', '/human-practices')}> Joshua </a>, we learned that CF statistics are inadequate, primarily representing the white population, which skews understanding of the disease's prevalence. We discovered that in Asian countries like Thailand, CF is underrepresented, leading to insufficient access to therapies and medications. </p>
<p>This awareness sharpened our focus on the need for improved science communication and highlighted the ongoing issues of racism and discrimination within scientific research. In response, we are committed to enhancing the data landscape in Germany by creating <a onClick={() => goToPageAndScroll ('our-surveys-on-cystic-fibrosis-and-gene-therapy', '/human-practices')}> surveys </a> in both German and English to gather broader insights and increase outreach. </p>
<figure>
<img src="link" alt="what is in the pic"/>
<figcaption>
......@@ -612,9 +615,10 @@ function AnalyseJoshua(){
)
}
function AnalyseWesthoff(){
const {goToPagesAndOpenTab} = useNavigation();
return(
<Collapsible title="Katrin Westhoff – Physiotherapist at local community " id="westhoffanalyseC">
<p>Katrin Westhoff’s expertise[Link] [HP_KatrinWesthoff(Physio).docx] in CF physiotherapy reinforced our approach to develop an inhalation-based therapy, especially for young patients. Her experience with children highlighted the need for a simple, accessible treatment, as younger patients may struggle with responsibility and consistency in therapy. This validated our decision to focus on inhalation delivery for ease of use. </p>
<p><a onClick={() => goToPagesAndOpenTab ('westhoffinv', '/human-practices')}> Kathrin Westhoff expertise </a> in CF physiotherapy reinforced our approach to develop an inhalation-based therapy, especially for young patients. Her experience with children highlighted the need for a simple, accessible treatment, as younger patients may struggle with responsibility and consistency in therapy. This validated our decision to focus on inhalation delivery for ease of use. </p>
<p>Additionally, we recognized through her insights that gene therapy, while impactful, is not a complete solution. Physiotherapy remains crucial in CF care, and our approach now integrates this understanding into a more holistic treatment plan. </p>
<div><img src="https://static.igem.wiki/teams/5247/photos/hp/besuch-westhoff/matte.webp" /></div>
<p><strong>Expertise in CF physiotherapy:</strong> Reinforced the importance of developing an inhalation-based therapy, especially for young CF patients.</p>
......@@ -631,10 +635,11 @@ function AnalyseWesthoff(){
)
}
function AalyseOlariu(){
const {goToPagesAndOpenTab} = useNavigation();
return(
<Collapsible title="Prof. Dr. Olariu – Physician and Clinical CF Expert " id="olariuanalyseC">
<p>Prof. Olariou's clinical insights shaped our project by emphasizing the complexities of CF treatment and the emotional burden on patients and families. His focus on early diagnosis, infection risks, and psychosocial impacts led us to design a solution that reduces the long-term care burden</p>
<p>Following his advice, we consulted psychologists[Link] (HP_Interview_Psychologinnen.docx ) to integrate mental health considerations and tailored our therapy to address different disease severities. Prof. Olariu also highlighted racial and global disparities in CF care, prompting us to explore these issues through further research and interviews. </p>
<p>Following his advice, we consulted <a onClick={() => goToPagesAndOpenTab ('psychol', '/human-practices')}> psychologists </a> to integrate mental health considerations and tailored our therapy to address different disease severities. Prof. Olariu also highlighted racial and global disparities in CF care, prompting us to explore these issues through further research and interviews. </p>
<div><img src="https://static.igem.wiki/teams/5247/integrated-human-practices/on-our-way-to-interview-psychologists.webp" alt=""/></div>
<p>His guidance on balancing treatment effectiveness with cost and overmedication concerns ensured our project remained patient-centered and practical. We also expanded our outreach efforts, raising CF awareness and collecting public data to align our project with community needs. </p>
<figure>
......@@ -662,11 +667,11 @@ function AalyseOlariu(){
}
function AnalyseMax(){
const {goToPagesAndOpenTab} = useNavigation();
return(
<Collapsible title="Max Beckmann – CF Patient and fellow student" id="maxanalyseC">
<p>Max's input as a cystic fibrosis (CF) patient directly influenced several key aspects of our project. After learning about the daily challenges of living with CF, we adapted our gene therapy approach to target the lungs, aligning our treatment with patient needs. His insights on the shortcomings of existing therapies strengthened our focus on developing a more effective solution.</p>
<p>Following Max's feedback, we implemented changes to our hygiene plan[Link], ensuring it meets the needs of immunocompromised individuals which we later presented to Mr. Johannfunke[Link], contact person for students with disabilities and impairments at the university of bielefeld, and checked its feasibility. Max’s perspective also shaped the content of our outreach materials, helping us portray CF in a more realistic and respectful way during the whole project. </p>
<p>Following Max's feedback, we implemented changes to our <a onClick={() => goToPagesAndOpenTab ('Patient MattersH', '/contribution')}> hygiene plan</a>, ensuring it meets the needs of immunocompromised individuals which we later presented to <a onClick={() => goToPagesAndOpenTab ('johannfunke', '/human-practices')}> Mr. Johannfunke</a>, contact person for students with disabilities and impairments at the university of bielefeld, and checked its feasibility. Max’s perspective also shaped the content of our outreach materials, helping us portray CF in a more realistic and respectful way during the whole project. </p>
<p>Additionally, Max contributed to the project by donating cells for our experiments, which allowed us to test our model systems effectively. Our ongoing communication with him has ensured that we stay patient-focused throughout, continually refining our approach based on his experiences. </p>
<div className="row">
<div className="col">
......
src/contents/engineering.tsx
+ 59
− 23
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src/sources/eng-peg-sources.tsx
+ 88
− 1
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......@@ -10,5 +10,92 @@ export default function EngPegsources(){
const bibtexSources = [
`
@article{Chow_Chen_Shen_Chen_2021,
title = {A web tool for the design of prime-editing guide RNAs},
author = {Chow, Ryan D. and Chen, Jennifer S. and Shen, Johanna and Chen, Sidi},
year = 2021,
month = feb,
journal = {Nature Biomedical Engineering},
publisher = {Nature Publishing Group},
volume = 5,
number = 2,
pages = {190–194},
doi = {10.1038/s41551-020-00622-8},
issn = {2157-846X},
rights = {2020 The Author(s), under exclusive licence to Springer Nature Limited},
abstractnote = {Prime editing enables diverse genomic alterations to be written into target sites without requiring double-strand breaks or donor templates. The design of prime-editing guide RNAs (pegRNAs), which must be customized for each edit, can however be complex and time consuming. Compared with single guide RNAs (sgRNAs), pegRNAs have an additional 3′ extension composed of a primer binding site and a reverse-transcription template. Here we report a web tool, which we named pegFinder (http://pegfinder.sidichenlab.org), for the rapid design of pegRNAs from reference and edited DNA sequences. pegFinder can incorporate sgRNA on-target and off-target scoring predictions into its ranking system, and nominates secondary nicking sgRNAs for increasing editing efficiency. CRISPR-associated protein 9 variants with expanded targeting ranges are also supported. To facilitate downstream experimentation, pegFinder produces a comprehensive table of candidate pegRNAs, along with oligonucleotide sequences for cloning.},
language = {en}
}
`,`
@article{Anzalone_Randolph_Davis_Sousa_Koblan_Levy_Chen_Wilson_Newby_Raguram_2019,
title = {Search-and-replace genome editing without double-strand breaks or donor DNA},
author = {Anzalone, Andrew V. and Randolph, Peyton B. and Davis, Jessie R. and Sousa, Alexander A. and Koblan, Luke W. and Levy, Jonathan M. and Chen, Peter J. and Wilson, Christopher and Newby, Gregory A. and Raguram, Aditya and Liu, David R.},
year = 2019,
month = dec,
journal = {Nature},
publisher = {Nature Publishing Group},
volume = 576,
number = 7785,
pages = {149–157},
doi = {10.1038/s41586-019-1711-4},
issn = {1476-4687},
rights = {2019 The Author(s), under exclusive licence to Springer Nature Limited},
abstractnote = {Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2–5. Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into a specified DNA site using a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase, programmed with a prime editing guide RNA (pegRNA) that both specifies the target site and encodes the desired edit. We performed more than 175 edits in human cells, including targeted insertions, deletions, and all 12 types of point mutation, without requiring double-strand breaks or donor DNA templates. We used prime editing in human cells to correct, efficiently and with few byproducts, the primary genetic causes of sickle cell disease (requiring a transversion in HBB) and Tay–Sachs disease (requiring a deletion in HEXA); to install a protective transversion in PRNP; and to insert various tags and epitopes precisely into target loci. Four human cell lines and primary post-mitotic mouse cortical neurons support prime editing with varying efficiencies. Prime editing shows higher or similar efficiency and fewer byproducts than homology-directed repair, has complementary strengths and weaknesses compared to base editing, and induces much lower off-target editing than Cas9 nuclease at known Cas9 off-target sites. Prime editing substantially expands the scope and capabilities of genome editing, and in principle could correct up to 89% of known genetic variants associated with human diseases.},
language = {en}
}
`,`
@article{Galietta_Haggie_Verkman_2001,
title = {Green fluorescent protein-based halide indicators with improved chloride and iodide affinities},
author = {Galietta, Luis J.V and Haggie, Peter M and Verkman, A.s},
year = 2001,
journal = {FEBS Letters},
volume = 499,
number = 3,
pages = {220–224},
doi = {10.1016/S0014-5793(01)02561-3},
issn = {1873-3468},
rights = {FEBS Letters 499 (2001) 1873-3468 © 2015 Federation of European Biochemical Societies},
abstractnote = {The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK a. However, a limitation of YFP-H148Q is its low halide sensitivity, with K d>100 mM for Cl−. Indicators with improved sensitivities are needed for cell transport studies, particularly in drug discovery by high-throughput screening, and for measurement of Cl− concentration in subcellular organelles. YFP-H148Q libraries were generated in which pairs of residues in the vicinity of the halide binding site were randomly mutated. An automated procedure was developed to screen bacterial colonies for improved halide sensitivity. Analysis of 1536 clones revealed improved anion sensitivities with K d down to 2 mM for I− (I152L), 40 mM for Cl− (V163S), and 10 mM for NO3 − (I152L). The anion-sensitive mechanism of these indicators was established and their utility in cells was demonstrated using transfected cells expressing the cystic fibrosis transmembrane conductance regulator chloride channel.},
language = {en}
}
`,`
@article{Bulcaen_Kortleven_Liu_Maule_Dreano_Kelly_Ensinck_Thierie_Smits_Ciciani_2024,
title = {Prime editing functionally corrects cystic fibrosis-causing CFTR mutations in human organoids and airway epithelial cells},
author = {Bulcaen, Mattijs and Kortleven, Phéline and Liu, Ronald B. and Maule, Giulia and Dreano, Elise and Kelly, Mairead and Ensinck, Marjolein M. and Thierie, Sam and Smits, Maxime and Ciciani, Matteo and Hatton, Aurelie and Chevalier, Benoit and Ramalho, Anabela S. and Casadevall i Solvas, Xavier and Debyser, Zeger and Vermeulen, François and Gijsbers, Rik and Sermet-Gaudelus, Isabelle and Cereseto, Anna and Carlon, Marianne S.},
year = 2024,
month = may,
journal = {Cell Reports Medicine},
pages = 101544,
doi = {10.1016/j.xcrm.2024.101544},
issn = {2666-3791},
abstractnote = {Prime editing is a recent, CRISPR-derived genome editing technology capable of introducing precise nucleotide substitutions, insertions, and deletions. Here, we present prime editing approaches to correct L227R- and N1303K-CFTR, two mutations that cause cystic fibrosis and are not eligible for current market-approved modulator therapies. We show that, upon DNA correction of the CFTR gene, the complex glycosylation, localization, and, most importantly, function of the CFTR protein are restored in HEK293T and 16HBE cell lines. These findings were subsequently validated in patient-derived rectal organoids and human nasal epithelial cells. Through analysis of predicted and experimentally identified candidate off-target sites in primary stem cells, we confirm previous reports on the high prime editor (PE) specificity and its potential for a curative CF gene editing therapy. To facilitate future screening of genetic strategies in a translational CF model, a machine learning algorithm was developed for dynamic quantification of CFTR function in organoids (DETECTOR: “detection of targeted editing of CFTR in organoids”).}
}
`,`
@article{Renz_Valdivia-Francia_Sendoel_2020,
title = {Some like it translated: small ORFs in the 5′UTR},
author = {Renz, Peter F. and Valdivia-Francia, Fabiola and Sendoel, Ataman},
year = 2020,
month = nov,
journal = {Experimental Cell Research},
volume = 396,
number = 1,
pages = 112229,
doi = {10.1016/j.yexcr.2020.112229},
issn = {0014-4827},
abstractnote = {The 5′ untranslated region (5′UTR) is critical in determining post-transcriptional control, which is partly mediated by short upstream open reading frames (uORFs) present in half of mammalian transcripts. uORFs are generally considered to provide functionally important repression of the main-ORF by engaging initiating ribosomes, but under specific environmental conditions such as cellular stress, uORFs can become essential to activate the translation of the main coding sequence. In addition, a growing number of uORF-encoded bioactive microproteins have been described, which have the potential to significantly increase cellular protein diversity. Here we review the diverse cellular contexts in which uORFs play a critical role and discuss the molecular mechanisms underlying their function and regulation. The progress over the last decades in dissecting uORF function suggests that the 5′UTR remains an exciting frontier towards understanding how the cellular proteome is shaped in health and disease.}
}
`,`
@article{Liang_He_Zhao_Zhu_Hu_Liu_Gao_Liu_Zhang_Qiu_2024,
title = {Prime editing using CRISPR-Cas12a and circular RNAs in human cells},
author = {Liang, Ronghong and He, Zixin and Zhao, Kevin Tianmeng and Zhu, Haocheng and Hu, Jiacheng and Liu, Guanwen and Gao, Qiang and Liu, Meiyan and Zhang, Rui and Qiu, Jin-Long and Gao, Caixia},
year = 2024,
month = jan,
journal = {Nature Biotechnology},
doi = {10.1038/s41587-023-02095-x},
issn = {1087-0156, 1546-1696},
url = {https://www.nature.com/articles/s41587-023-02095-x},
language = {en}
}
`
]
\ No newline at end of file
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