<Subesctiontitle="Combining Primeguide and AirBuddy"id="Experimental Design6">
<H5text="Transfection"/>
<divclassName='col'>
<p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection ofPE6cpegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>
<p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection of pPCMV-PE6c and pegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>
</div>
<divclassName='col'>
<OneFigure
...
...
@@ -576,7 +576,7 @@ export function Results() {
bg="white"
description="Microscopy of CFBE4o- 48h aftr transfection. Cells were previoulsy transfectet with pPEAR_CFTR and later tresnfected with PE6c+pegRNA4 via AirBuddy. BF = Brightfield "
/>
<p>The results show a sucsessful transfection of PE6cpegRNA4 encapsulated in our LNPs was achieved following prior transfection with pPEAR_CFTR. The experimental setup demonstrated a significant increase in fluorescence intensity, indicative of enhanced Prime Editing efficiency under these conditions.
<p>The results show a sucsessful transfection of pCMV-PE6c and pegRNA4 encapsulated in our LNPs was achieved following prior transfection with pPEAR_CFTR. The experimental setup demonstrated an increase in fluorescence intensity, indicative of enhanced Prime Editing efficiency under these conditions.
Moreover, the PreCyse results confirm the effectiveness of our strategy, showcasing the synergy between our proprietary PrimeGuide system and the AirBuddy platform. This combination has proven to be pivotal in optimizing the editing outcomes and advancing the precision of the Prime Editing process.
These findings underscore the robustness of our delivery approach and the innovative integration of the PrimeGuide and AirBuddy systems in driving Prime Editing to new levels of efficiency and accuracy.</p>