Merge branch 'main' of ssh://gitlab.igem.org/2024/bielefeld-cebitec

src/contents/Human Practices/Further Engagement/Education.tsx

@@ -124,12 +124,12 @@ Moreover, we connected with other institutions and participants at the event. We
             <H4 id="mint-heading" text="MINT Sommer"/>
                 <H5 id="what and why mint summer" text="What is MINT Summer and why were we participating?"/>
                     <p>“MINT Summer 2024” is a comprehensive program designed primarily for high school graduates of the class of 2024, who are considering pursuing studies in STEM fields (Science, Technology, Engineering, and Mathematics, including teaching degrees). The program is perfect for those who are still uncertain if they want to study in STEM or which specific discipline aligns best with their interests.</p>
-                    <p>Our participation in “MINT Summer” offered us the chance to raise awareness of cystic fibrosis and showcase our cutting-edge approach to develop an optimized gene therapy to combat this disease. Through the event we engaged with potential future scientists and researchers, informing them about our project, iGEM and the importance of scientific research in advancing medical treatments. This program not only allows us to share our mission but also to inspire the next generation of STEM students by highlighting the real-world impact of their studies. </p>
+                    <p>Our participation in <a href="https://www.uni-bielefeld.de/studium/studieninteressierte/mint-sommer/" title="Mint Summer" >MINT Summer </a> offered us the chance to raise awareness of cystic fibrosis and showcase our cutting-edge approach to develop an optimized gene therapy to combat this disease. Through the event we engaged with potential future scientists and researchers, informing them about our project, iGEM and the importance of scientific research in advancing medical treatments. This program not only allows us to share our mission but also to inspire the next generation of STEM students by highlighting the real-world impact of their studies. </p>
                 <H5 id="strategy summer" text="What was our strategy?"/>
                     <p>Our objective at MINT Summer was to inform attendees, especially aspiring STEM students, about the unique challenges faced by cystic fibrosis (CF) patients, with a particular focus on lung-related complications. We drew heavily on the insights gained from the Science Communication Workshop at the BFH Meetup, which provided us with the perfect framework to meticulously plan our outreach for this event. This foundation allowed us to craft engaging and educational activities that effectively conveyed the complexities of CF to our audience, ensuring our message was both impactful and accessible. </p>
                     <p>We took the opportunity to explain the iGEM competition and our project to participants. We shared how iGEM is a global competition that brings together student teams to solve real-world problems using synthetic biology. We discussed how our approach aims to correct the genetic mutation responsible for CF, potentially offering a more effective treatment. By engaging with attendees, we were able to highlight the significance of our research and the impact it could have on improving the lives of those affected by this challenging condition. They got the opportunity to contribute to our project by participating in our survey. </p>
                     <p>Over the time of two weeks, we established meaningful connections with professors, students, and participants across various STEM fields during the event, like the student initiative btS and the Campusbrauerei Bielefeld. Sharing our project with the life science students was helpful, motivating and opened the door to engaging discussions that enriched our perspective and fostered collaboration. These interactions allowed us to connect with experts and students from different disciplines, enhancing our understanding of how our gene therapy research for cystic fibrosis fits within the broader scientific landscape.</p>
-                <H5 id="coclusion summer" text="What is our conclusion?"/>
+                <H5 id="conclusion summer" text="What is our conclusion?"/>
                     <p>The experience allowed us to refine our science communication skills and connect with a broad range of STEM professionals and students. Overall, the event was a valuable opportunity for both education and professional growth, making it a rewarding and impactful experience for our team. </p>
             </div>
 

src/contents/Human Practices/Further Engagement/Outreach.tsx

@@ -48,7 +48,7 @@ export function HPOutreach(){
             <p>The waffle sale was a great success, both in terms of raising funds and increasing awareness about our work within the university. It was a collaborative effort that brought our team closer together and demonstrated the power of community support in advancing scientific research. </p>
             </div>
             <div className="col-4"> 
-              <img src="https://static.igem.wiki/teams/5247/photos/edcation-and-outreach/screenshot-2024-09-25-202008.png" style={{objectFit: "cover", maxHeight: "60%", width: "100%"}}/> 
+              <img src="https://static.igem.wiki/teams/5247/photos/edcation-and-outreach/screenshot-2024-09-25-202008.png" style={{objectFit: "cover", maxHeight: "40%", width: "100%"}}/> 
           </div>
           </div>
           

src/contents/methods.tsx

 import { Section } from "../components/sections";
 import { useTabNavigation } from "../utils/TabNavigation";
+import {H4, H2} from "../components/Headings";
 
 export function Methods() {
   useTabNavigation();
@@ -12,7 +13,14 @@ export function Methods() {
           ...
         </Section>
         <Section title="Cell Lines" id="Cell Lines">
-          ...
+          <H2 text="Used Cell lines"></H2>
+          <H4 text="HEK293 and HEK293T cell lines"></H4>
+          <p>For testing our prime editing approach, we needed an easy-to-handle cell line with a measurable high expression of CFTR and the CFTR F508del mutation. When talking to Mattijs Bulcaen from the Laboratory of Molecular Virology and Gene Therapy at KU Leuven, he recommended to use HEK293T cell lines overexpressing CFTR they had used. HEK293 cells are a very common immortalized human cell line derived from the kidneys of a female embryo. They are particularly suited to research due to their convenient handling and transfection properties. Basic HEK293 cells were provided to us by the Cellular and Molecular Biotechnology working group at Bielefeld University led by Prof. Dr. Kristian Müller, who is also one of the Principal Investigators of our team. HEK293T cells express an additional tsA1609 allele of the SV40 large T-antigen, allowing for replication of vectors containing the SV40 origin of replication.[2] Besides the native CFTR gene, which is not expressed in HEK cells, the HEK293T cell lines used in Leuven carry another copy of the gene embedded in an expression cassette. The cassette includes a CMV promoter, which is a standard promoter used for gene overexpression in human cells derived from the human Cytomegalovirus[4], as well as a puromycin resistance co-expressed with the CFTR allowing for continuous selection of CFTR expressing cells. The whole construct was stably inserted into the genome using lentiviral transduction.1,3 </p>
+          <H4 text="CFBE41o- cell line "></H4>
+          <p>The CFBE41o- cell line, derived from bronchial epithelial cells of a one-year-old cystic fibrosis patient, serves as a vital model for studying cystic fibrosis. These cells closely mimic the physiological environment of the airway epithelium, allowing for more accurate studies on how CFTR mutations affect cell function and response to treatments. They were immortalized through calcium-phosphate-mediated transfection using a replication-defective pSVori plasmid that carries the simian virus 40 large T-antigen (SV40-LT). The plasmid's defective origin of replication prevents viral propagation, thus preserving essential physiological characteristics of the cells while enabling them to develop differentiated morphologies. CFBE41o- cells are homozygous for the ΔF508-CFTR mutation [1]. We are happy we got this cell line with permission from Prof. Dr. Zoya Ignatova, who is leader of a working group at the Institute for Biochemistry and Molecular Biology of Hamburg University and an iGEM supporter since a long time [6]. </p>
+          <H4 text="Human nasal epithelial cells (hNECs)"></H4>
+          <p>Human nasal epithelial cells were obtained by nasal brushing, a minimally invasive method. These cells function/act as primary cultures. Cultivated in air-liquid interface (ALI) cultures and apical-out airway organoids (AOAO), they serve as a suitable model to visualise the functional epithelium of the airways in a differentiated form. The in vivo aspects of an airway disease, such as CF, can be modelled using donors with those airway diseases (5) This model is therefore particularly suitable for testing our prime editing complex. </p>
+
         </Section>
       </>
     );