added introduction for PoC Engineering cycle

src/contents/engineering.tsx

@@ -38,13 +38,13 @@ export function Engineering() {
                <section  >
                   <div className="bg-lb box" >
                      <H3 id="Proof of Concept" text="Proof of Concept"></H3>
-                     <p>To test prime editors, a reliable model system is requeired. HEK293 cells are a human derived cell line and widely used in </p>
+                     <p>To test prime editors, a reliable model system is required. HEK293 cells are a human derived cell line and widely used in a variety of fields in biology[1]. Apart from easy handling and comparatively easy transfection, they have, as we found out in our exchange with Mattijs Bulcaen, one advantage over other models: They are naturally impaired in DNA repair mechanisms and therefore easier to edit. To properly compare editing efficiencies, a high transfection efficiency is of utmost importance. This engineering cycle focuses on our work in simulating prime editing using the PEAR reporter system[2] and optimizing transfection protocols.</p>
                   </div>
                   <div className="box" >
                      <p id="cyc1">
                         <H3 text="Test of Lipofectamine 2000" id="text"/>
                         <H4 text="Test" id="text"/>
-                        <p>While conducting research on transfection methods for HEK cells, particular attention was devoted to the delivery of the Prime Editing complex into the cells. In the literature, Lipofectamine is described as a common transfection agent. Anzalone et al. 2019 describe a transfection of prime-editing complexes with Lipofectamine 2000. The aim of this study is to introduce our prime-editing complex into HEK cells.</p>
+                        <p>While conducting research on transfection methods for HEK cells, particular attention was devoted to the delivery of the Prime Editing complex into the cells. In the literature, Lipofectamine is described as a common transfection agent. <i>Anzalone et al. 2019</i>[3] describe a transfection of prime-editing complexes with Lipofectamine 2000. The aim of this study is to introduce our prime-editing complex into HEK cells.</p>
                         <p>Transfection with Lipofectamine 2000 was performed in accordance with the Anzalone protocol. However, the result was characterized by insufficient transfection efficiency.</p>
                         <H4 text="Learn" id="text"/>
                         <p>The low efficiency of Lipofectamine 2000 indicated that the product is not optimally suited to the specific conditions under consideration. In contrast, Lipofectamine 3000 is described in the literature as potentially more efficient.</p>
@@ -57,7 +57,7 @@ export function Engineering() {
                   </div>
                   <div className="box" >
                      <p id="cyc2">
-                        <H3 text="Initial Test woth Lipofectamine 3000" id="text"/>
+                        <H3 text="Initial Test with Lipofectamine 3000" id="text"/>
                         <H4 text="Test" id="text"/>
                         <p>Considering the favorable assessment of Lipofectamine 3000 in the scientific literature, the proof of concept was conducted once more.</p>
                         <p>The objective of the experiment was to enhance the transfection efficiency of Lipofectamine 3000. The transfection protocol was conducted in accordance with the manufacturer's instructions (1 µg DNA, 2 µl Lipofectamine 3000 reagent).</p>