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Commit 4a3fc774 authored by Kathleen Susat's avatar Kathleen Susat
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pics test mech results

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......@@ -6,6 +6,7 @@ import { useTabNavigation } from "../utils/TabNavigation";
import { H5 } from "../components/Headings";
import { useNavigation } from "../utils";
import { DownloadLink } from "../components/Buttons";
import { TwoHorizontal } from "../components/Figures";
export function Results() {
......@@ -24,12 +25,7 @@ export function Results() {
<H4 text="Proof-of-concept 1"/>
<H5 text="Workflow"/>
<p>The prepared pDAS12124-preedited plasmid serves as a positive control to validate the success of the experiment. A technical control with the pZMB938 plasmid confirms successful transfection of the cells. In the main part of the experiment, pDAS12489-2in1 and pCMV-PE2 are co-transfected. Successful transfection is visualised by GFP signals.</p>
<div className="figure-wrapper">
<figure>
<img src="" style={{height: "10%", width: "auto"}}/>
<figcaption> <b>Figure 1</b> XXXX.</figcaption>
</figure>
</div>
<H5 text="Conclusion"/>
<p>The microscopy data validates our proof of concept. Compared to our internal positive control, pDAS12124-preedited (see Figure X), less cells co-transfected with pDAS12489 and pCMV-PE2 (see Figure X) showed fluorescence. Contrary to our expectations, the technical transfection control with pZMB938 showed lower transfection efficiency. All negative controls showed no fluorescence.</p>
<H4 text="Proof-of-concept 2"/>
......@@ -63,7 +59,13 @@ export function Results() {
<H5 text="Workflow"/>
<p>With this experiment we wanted to compare the efficiency of pegRNAs with and without silent edits.</p>
<H5 text="Conclusion"/>
<p>The FACS analysis shows that pegRNA without silent edits (pegRNA1) had a 2.05 times higher transfection efficiency than pegRNA with silent edits (pegRNA2).</p>
<p>The FACS analysis shows that pegRNA without silent edits (pegRNA1) had a 2.05 times higher transfection efficiency than pegRNA with silent edits (pegRNA2).</p>
<TwoHorizontal
description="FACS analysis of pegRNAs with and without silent edits."
num={1}
pic1="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/se-nose.png"
pic2="https://static.igem.wiki/teams/5247/photos/facs-results-mechanism/bild8.png"
/>
<H4 text="pegRNA 2"/>
<H5 text="Workflow"/>
<p>Cotransfection of pPEAR_CFTR and PE2 and also 1 of the 14 pegRNAs to compare the transfection efficiency of all of our designed pegRNAs.</p>
......
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