heading:"Improvement of our culture protocols and experimental outcomes, particularly in maintaining ALI cultures",
interviewtabid:"pkuehnel",
cardtext:"",
quote:"x",
quote:"Connecting with Philipp Kühnel, an expert in nasal cell cultivation, has been invaluable. His insights and expertise in ALI techniques have significantly enhanced our understanding and approach in this crucial area of research.",
quoteNachname:"Susat, Teammember ",
quoteVorname:"Kathleen",
aimofcontact:[<p>The aim of our contact with Philipp Kühnel, a PhD student from the Otorhinolaryngology working group of Bielefeld University, was to gain expertise in working with primary cultures, particularly focusing on air-liquid interface (ALI) cultures. Given his experience in this area, we sought his guidance to ensure that we were following best practices and to address any technical challenges we might encounter.</p>],
insights:[<p>Through our discussions with Philipp, we gained valuable insights into the optimal conditions for cultivating primary cells and maintaining ALI cultures. He provided practical advice on troubleshooting of common issues, such as cell differentiation and culture stability, which were crucial for the success of our experiments. We also maintained close contact to exchange information about fungi that frequently contaminate ALI cultures. The expertise shared on combating these fungal contaminations was particularly valuable and greatly enhanced our understanding of effective prevention and treatment methods. </p>],
implementation:[<p>We incorporated Philipp’s advice by refining our culture protocols, particularly adjusting the conditions for ALI cultures to improve cell differentiation and overall culture health. This directly enhanced the reliability of our experimental results, ensuring that our work with primary cultures was both accurate and reproducible. </p>],