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Commit 0d9ada63 authored by Vera Köhler's avatar Vera Köhler
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Again Änderungen PreCyse Results

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...@@ -566,7 +566,7 @@ export function Results() { ...@@ -566,7 +566,7 @@ export function Results() {
<Subesction title="Combining Primeguide and AirBuddy" id="Experimental Design6"> <Subesction title="Combining Primeguide and AirBuddy" id="Experimental Design6">
<H5 text="Transfection"/> <H5 text="Transfection"/>
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<p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection of pPCMV-PE6c and pegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p> <p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection of pCMV-PE6c and pegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>
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<OneFigure <OneFigure
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