<Subesctiontitle="Combining Primeguide and AirBuddy"id="Experimental Design6">
<Subesctiontitle="Combining Primeguide and AirBuddy"id="Experimental Design6">
<H5text="Transfection"/>
<H5text="Transfection"/>
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<p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection of pPCMV-PE6c and pegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>
<p>Fluorescence microscopy was performed using the Leica DMI6000 B microscope at 20x magnification to analyze CFBE4o- cells. These cells were initially pre-transfected with pPEAR_CFTR to establish a baseline for Prime Editing activity. Subsequently, transfection of pCMV-PE6c and pegRNA4, delivered via the AirBuddy system, was carried out. LNPs without cargo acted as the negative control. Imaging was conducted at 24h, 48h, and 72h post-transfection to assess fluorescence intensity and evaluate editing efficiency over time.</p>