<QaBoxq="No,not yet."a="Because they had a paper in science where they did intravenous delivery of lipid nanoparticles and they, they delivered a base editor. So when you present this, this might come up. So, you know, so I'm not saying you should change your strategy, but, you know, if you're trying to do a lot of work on lipid nanoparticles, you're going to have to do a lot of work on So you want to have thought about a response. And it's, it's a really interesting strategy. Um, trying to see if I can find it. Yeah. Here. It's a really interesting sort of nanoparticle strategy that they have presented. Uh, the nice thing is it is an IV delivery, which gets you to basal cells and they were able to get about 20% correction, right? So you could almost think of it as an alternative strategy. So if, you know, if you get the pushback or I don't know how you have to write to your proposal, but if the, the, the spray drying or the nebulization process is not going well for you, this might be something that you could propose as a backup because here they've shown that you can deliver lipid nanoparticles intravenously and they get to the basal cell population. Okay. And they get to, yeah. So that might be the only other direction I would point to as you're thinking about your delivery strategy. Right. Uh, the other innovation could be that if you can even optimize your prime editing to go a little bit beyond just Delta, if I wait, if you can show that you even replace the whole Exxon, you might have something that is a little bit more unique than what's out there. And then you don't have to even worry about delivery necessarily. "/>
<QaBoxq="Okay. Thank you so much. We will look at the paper and we'll check it out. All right. Okay. Then, yeah. Thank you very much for the interview. "a="Yeah, that's perfectly fine. Good luck with your project. It's a really interesting project."/>
heading:"Performance of Experiments for LNP characterization ",
interviewtabid:"biophysik",
cardtext:"",
quote:"x",
quote:"It was a great connection and a tremendous help in analyzing the LNP. They provided us with insights into some truly exciting methods.",
quoteNachname:"Baack, Teammember",
quoteVorname:"Anna",
aimofcontact:[<p>For our project, we collaborated closely with the Physical Chemistry workgroup to properly categorize our lipid nanoparticles (LNPs). We reached out to them to leverage their expertise and ensure that our characterization was thorough and precise. Marco, Uwe, and Yvonne were instrumental in this effort, not only advising us on appropriate characterization methods but also actively assisting us during the experimental process and data analysis. </p>],
insights:[<p>We employed several analytical techniques, including Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and zeta potential analysis. TEM and SEM allowed us to visualize the structural morphology of the LNPs, providing detailed images to understand their size and shape on the nanometer scale. DLS was used to measure the size distribution of the particles in solution, while the zeta potential analysis gave us insight into the surface charge, which is crucial for understanding stability in suspension. </p>],
implementation:[<p>Thanks to the guidance and hands-on support of the Physical Chemistry team, we successfully completed these tests, gaining detailed insights into our LNPs that will be crucial for our project's further development. Their expertise not only streamlined the process but also ensured the reliability and accuracy of our results. Here a sneak peak of the results – take a look at the image of our SORT LNP taken via TEM. </p>],
quote:"Chitosan can stabilize RNA effectively, making it ideal for our formulations, but we must ensure the right charge ratio and particle size for successful LNP encapsulation.",
quoteNachname:"Moorlach, Expert for Chitosan coding",
quoteVorname:"Benjamin",
aimofcontact:[<p>The aim of the contact with Benjamin Willem Moorlach, M.Sc., from the Department of Engineering and Mathematics, was to gain a deeper understanding of how Chitosan could be applied in lipid-based nanoparticles (LNPs) and to explore its potential role in our project. We had several questions focusing on the properties of Chitosan, its advantages and disadvantages, and how it could be integrated into LNPs. Benjamin Moorlach provided extensive insights into Chitosan’s interactions with RNA, its behavior, and how we might leverage it for our formulations. </p>],
insights:[<p>From our discussion, we gained valuable insights into the unique properties of Chitosan, a cationic polymer with significant potential to stabilize RNA. Notably, Chitosan offers strong protection against RNases, making it highly beneficial for formulations like lipid-based nanoparticles (LNPs). Another key feature is its heat stability, withstanding temperatures up to 121°C, which makes it suitable for processing methods such as spray drying. However, at higher concentrations (0.5% or more), Chitosan can become toxic, suffocating cells and displaying antimicrobial properties. While it differs from PEG and cannot serve as a direct alternative, Chitosan can be a valuable complement, especially in stabilizing RNA within LNPs.
A critical point Benjamin emphasized is that Chitosan must be in an acidic environment, typically with a pH range of 4 to 6, to remain positively charged. This positive charge is essential for its effective interaction with RNA and successful integration into the LNP system.
