<divclass=" myPage-paragraph-fig-description"><b>Fig. 38 Co-culture results of these two
engineered
bacteria. a</b> co-culture broth. <b>b</b> sediment after centrifugation.
</div>
</div>
<h5class="myPage-paragraph-headline-h5"><b>Co-culture fermentation in hardware</b></h5>
<pclass="myPage-paragraph-content">
Two cycles of repeated fed-batch were carried out in hardware to ensure the functionality of hardware. The crosslinked compound of bacterial cellulose and hyaluronic acid produced successfully, and the product was filtered out to be used in further analysis.
Two cycles of repeated fed-batch were carried out in hardware to ensure the functionality of hardware. The crosslinked compound of bacterial cellulose and hyaluronic acid produced successfully (as shown in Fig. 39), and the product was filtered out to be used in further analysis.
<divclass="myPage-paragraph-fig-description"><b>Fig. 39 The outlook of the harvested crosslinked compound from repeated fed-batch fermentation.
</div>
<pclass="myPage-paragraph-content">
We conducted an Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) assay to confirm whether the crosslinking occurred. As shown in Fig. 38, a peak at 1028 cm<sup>-1</sup> can be observed, indicates that the crosslinking between bacterial cellulose and hyaluronic acid occurred during co-culture fermentation.
We conducted an Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) assay to confirm whether the crosslinking occurred. As shown in Fig. 40, a peak at 1028 cm<sup>-1</sup> can be observed, indicates that the crosslinking between bacterial cellulose and hyaluronic acid occurred during co-culture fermentation.
<divclass="myPage-paragraph-fig-description"><b>Fig. 40 ATR-FITR diagram, comparing the characteristic of harvested crosslinked product and standard compound.
</div>
<pclass="myPage-paragraph-content">
Also, a water retention ability test in soil was carried out with the bacterial culture containing the crosslinked product. As shown in Fig. 39, the bacterial culture has greater water retention ability than pure water, indicates that our product produced in the hardware functions well as a water retention material which can maintain the moisture of soil.
Also, a water retention ability test in soil was carried out with the bacterial culture containing the crosslinked product. As shown in Fig. 41, the bacterial culture has greater water retention ability than pure water, indicates that our product produced in the hardware functions well as a water retention material which can maintain the moisture of soil.
<divclass="myPage-paragraph-fig-description"><b>Fig. 41 A diagram showing the result of water retention ability of the harvested crosslinked compound in soil.
