<h4> The Restriction Barrier in Cyanobacteria Chassis</h4>
<p>Cyanobacteria synthetic biology has broad applications ranging from pharmaceutical production to carbon capture and bioremediation [1]. Regardless of the great potential of cyanobacteria chassis, engineering them remains difficult in part due to the presence of restriction-modification (R-M) systems that target foreign DNA, lowering transformation efficiency [2]. Despite awareness of this barrier to transformation and the development of techniques to avoid R-M systems in cyanobacteria, R-M systems continue to make engineering cyanobacteria a difficult task [2]. To improve the situation, we are developing a software pipeline for avoiding the presence of restriction sites on foreign DNA to be introduced into a cyanobacteria chassis, thereby making cyanobacteria easier to engineer.</p>
