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Commit 8ddffada authored by Lucy Hao's avatar Lucy Hao :moyai:
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......@@ -333,16 +333,6 @@ density in liquid cultures and adjusted the pH similar to sea level. The
goal was to increase plasmid extraction yields in preparation for the
cell extract production.
### T7 promoter improvement
Previous literature has shown that protein expression in CFPS is more
rapid from endogenous promoters relative to the T7 promoter. Thus, we
designed an improved T7 promoter that contains adjacent -10 and -35
sites, as well as an UP element. The promoter is regulated by flanking
lacO sites to prevent expression of toxic genes until properly induced.
We cloned this promoter into our expression vector using Gibson
assembly.
## Future Directions
Although we were unable to characterize our Expression Vector via
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......@@ -10,9 +10,7 @@ export const meta = {
<div className="padded-content">
## Safety in the Lab
Since our team worked extensively with E. coli and V. natriegens, we took many
measures to ensure safety in the lab. The lab we worked in was classified as
**Containment Level 2** and we worked with **Risk Group 1 Biological
Since our team worked extensively with *E. coli* and *V. natriegens*, we took many measures to ensure safety in the lab. The lab we worked in was classified as **Containment Level 2** and we worked with **Risk Group 1 Biological
Material**.
<Figure
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