@@ -321,6 +321,15 @@ A Gibbs free energy value of 11249.38 +/- 70.50 kJ/mol (2688.666348 cal/mol) ind
Thus, this should be taken into account when designing experiments with the Staygold protein, and perhaps another protein of choice may be better suited.
@@ -377,7 +386,7 @@ To begin, we identified all exchange reactions present in the *V. natriegens* GS
Next, we tested different uptake rates for each metabolite by relaxing its lower bound constraint , and classified them as either having an impact on the overall Biomass Accumulation Rate (BAR) or not. Solutions to the FBA were calculated using COBRA’s _optimizeCbModel()_ function, and solutions being visualized using MATLAB’s base _plot()_ function.
Results revealed that only **Glucose and Fructose** substantially affected overall BAR. Among the remaining carbon sources, **D-Mannitol and Acetate** caused minor fluctuations in the BAR, while Glycerol, D-Mannose, Dulcose, Amylotriose, and Matohexaose had no effects on the BAR.
Results revealed that only **Glucose and Fructose** substantially affected overall BAR. Among the remaining carbon sources, **D-Mannitol and Acetate** caused minor fluctuations in the BAR, while Glycerol, D-Mannose, Dulcose, Amylotriose, and Matohexaose had no effects on the BAR.
<Figure src="https://static.igem.wiki/teams/4796/wiki/mathmodelling/1.png" num="8" caption={<>Plots showing the effect of varying uptake rate of <strong>only Glucose and Fructose</strong> on the BAR. The optimal Fructose uptake rate is 650 mmol/day, allowing for a growth rate of 44.23 1/day. The optimal Glucose uptake rate is 980 mmol/day, allowing for a growth rate of 36.58 1/day.</>}/>