Download the zip file and then extract all files. If you are using windows then please use the windowsrun command. If using mac then use the maccommandrun. The program runs off of java and models the process of optimizing a lead sequence for the type 3 secretion system in order to achieve maximum biological activity. Please have your DNA sequence (up to 20 nucleotides) and activity value for your DNA sequence being secreted by the T3SS ready as the user inputs this to get the program started.