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Commit be4f2e38 authored by Xinyi Zhang's avatar Xinyi Zhang
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......@@ -311,11 +311,13 @@ document that contribution.{% endblock %} {% block header %}
alt=""
width="50%"
/>
<figcaption><b>Fig.4 | </b>The apperance of our hardware</figcaption>
<img
src="https://static.igem.wiki/teams/4583/wiki/contribution/1-2.png"
alt=""
width="80%"
/>
<figcaption><b>Fig.5 | </b>The app operation interface</figcaption>
</div>
</div>
<!-- 04 -->
......@@ -346,7 +348,7 @@ document that contribution.{% endblock %} {% block header %}
width="80%"
/>
<figcaption>
<b>Fig.4 | </b>Binding energy of proteins with AHL.
<b>Fig.6 | </b>Binding energy of proteins with AHL.
</figcaption>
</div>
</div>
......@@ -364,12 +366,11 @@ document that contribution.{% endblock %} {% block header %}
</p>
<div>
<img
src="https://static.igem.wiki/teams/4583/wiki/contribution/the-circular-feedback-process-of-our-questionnaire.png"
alt="The circular feedback process of our questionnaire."
src="https://static.igem.wiki/teams/4583/wiki/hp/the-circular-feedback-process-of-our-questionnaire.png"
width="80%"
/>
<figcaption>
<b>Fig.5 | </b>The circular feedback process of our questionnaire.
<b>Fig.7 | </b>The circular feedback process of our questionnaire.
</figcaption>
</div>
<p>
......
......@@ -4,6 +4,10 @@
.dsc-bg {
background-image: url("https://static.igem.wiki/teams/4583/wiki/modeling/ep-pic-banner01.png");
}
.dis img {
display: block;
margin: 0 auto;
}
</style>
<div class="inp-bg dsc-bg">
<div class="inp-title">
......@@ -55,6 +59,9 @@
<a href="#tl"
><p><strong>Three-layer dynamic regulation model</strong></p></a
>
<a href="#ach"
><p><b>Achievement</b></p></a
>
<a href="#fd"
><p><b>Further development</b></p></a
>
......@@ -66,7 +73,7 @@
<div id="progress-bar"></div>
</div>
</div>
<div class="col-sm-9 wiki-content">
<div class="col-sm-9 wiki-content dis">
<div>
<div>
<ul class="gd-t1">
......@@ -97,7 +104,7 @@
<p>
Because of its advantages such as durability, flexibility, and
affordability, <b>plastic</b> has become an important part of our
lives[1]. Traditional petroleum-based plastics typically include
lives. Traditional petroleum-based plastics typically include
polypropylene (PP), polystyrene (PS), polyethylene terephthalate
(PET), polyvinyl chloride (PVC), low density polyethylene (LDPE), or
high-density polyethylene (HDPE), which are
......@@ -148,14 +155,7 @@
alt="Fig.2 The first part of our Human Practice: Plastic pollution and us. Our initial human practice was mainly divided into three parts: sampling and analysis, questionnaire and interviews."
width="75%"
style="display: block; margin: 0 auto"
/>
<br />
<img
src="https://static.igem.wiki/teams/4583/wiki/project-description/2.jpg"
alt="Fig.2 The first part of our Human Practice: Plastic pollution and us. Our initial human practice was mainly divided into three parts: sampling and analysis, questionnaire and interviews."
width="75%"
style="display: block; margin: 0 auto"
/>
/>
<figcaption>
<b>Fig.2 | </b> The first part of our Human Practice: Plastic
pollution and us. Our initial human practice was mainly divided into
......@@ -193,7 +193,7 @@
/>
<figcaption><b>Fig.3 | </b> PHB granule in the cell</figcaption>
<p>
<b>PHB (poly-β-hydroxybutyrate)</b> is a class of PHAs[2]. PHB
<b>PHB (poly-β-hydroxybutyrate)</b> is a class of PHAs. PHB
possesses better <b>physical properties</b> than polypropylene for
applications such as food packaging and is completely
<b>nontoxic</b>.
......@@ -364,7 +364,7 @@
turn off PesaR and turn on P<sub>esaS</sub>. In the presence of AHL,
EsaR can bind to AHL and release from the DNA. Thus,
<b>at high cell density(high ρ)</b>, the P<sub>esaR</sub> is turned
on and the P<sub>esaS</sub> is turned off<sub>[3]</sub>.
on and the P<sub>esaS</sub> is turned off<sub></sub>.
</p>
</div>
......@@ -386,7 +386,7 @@
</figcaption>
<p>
We will use <b>the lambda phage lysis gene cassette</b> to design
our auto-lysis system<sub>[4, 5]</sub>.
our auto-lysis system<sub></sub>.
</p>
</div>
......@@ -429,6 +429,38 @@
>, the promoter will turn on and then <b>the cell lysis</b>.
</p>
</div>
<div>
<br />
<hr
style="height: 5vh; border: none; border-top: 5vh groove skyblue"
/>
<br />
<h2 style="text-align: center">
<a name="" ></a>
</h2>
<br />
<P>We obtained <b>ten valid results</b> from the experimental construction as follows</P>
<div>
<img src="https://static.igem.wiki/teams/4583/wiki/team/discription/the-characterization-results-of-three-layer-dynamic-regulation-model1.png"
alt="" width="80%">
<img src="https://static.igem.wiki/teams/4583/wiki/team/discription/the-characterization-results-of-three-layer-dynamic-regulation-model2.png"
alt="" width="80%">
<figcaption><b>Fig.10 | </b>The characterization results of Three-layer dynamic regulation model.</figcaption>
</div>
<p><b>An example</b>: we can see from it that its three stages successfully achieved <b>separation</b>.</p>
<div>
<img src="https://static.igem.wiki/teams/4583/wiki/team/discription/l19-rwt-pyu3.png" alt="" width="60%">
<figcaption><b>Fig.10 | </b>The model of L19-PesaRwt-PYU3</figcaption>
</div>
<p>
We applied our model to PHB production and found that the extracellular PHB content of the model with the three-layer dynamic regulation model was <b>much higher</b> than that of the one without the complete three-layer dynamic regulation model.
</p>
<div>
<img src="https://static.igem.wiki/teams/4583/wiki/team/discription/re.png" alt="" width="60%">
<figcaption><b>Fig.11 | </b>Results</figcaption>
</div>
</div>
<div>
<ul class="gd-t1">
<a name="fd"> <br /></a>
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</div>
<!-- reference -->
<div>
<div>
<ul class="gd-t3">
<a name="ref"> <br /></a>
<li>Refences</li>
</ul>
</div>
<div class="table">
<ul class="table-text" style="margin-left: 0">
<li><p>References</p></li>
......@@ -1699,7 +1699,7 @@ endblock %} {% block header %}
/>
</div>
<div id="table-re" class="table-wrapper hide">
<table class="table-fo">
<table class="table-fo" id="reference">
<tr>
<td>
<p>
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