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<li>The mosquito, responsible for more than 750,000 annual deaths, has earned the distinction of being the most lethal creature on Earth. Its deadly reputation, however, stems not from its size or predatory nature, but rather from its role as a carrier for numerous diseases. For example, the tiger mosquito Aedes albopictus is specifically known for its aggressive biting behavior and ability to transmit various diseases, including Chikungunya, Dengue fever, and Zika virus.
<li>Our decision to focus on mosquitoes come from the significant challenges they pose in our region, making them a pressing concern and a prominent research subject. Numerous teams from different institutes have expressed their interest in this area, motivating us to develop a detection method that can streamline research efforts and contribute to the field.
Inspired by the work of our predecessors, we have adopted the SHERLOCK technique as our foundation. This approach utilizes Cas nucleases combined with a guide sequence that complements the viral RNA. When the viral sequence is identified, the Cas nuclease not only cleaves it but also cleaves the surrounding region, resulting in the release of a fluorescent molecule called FAM. Detection of FAM fluorescence serves as an indicator of viral presence.
In the initial phase, we embarked on a comparison between two Cas nucleases: the one employed by last year's team, Cas13a and a novel protein, CasRX, chosen by our own team. We evaluated their efficiency at various concentrations of viral sequences that we designed and had synthesized from Chikungunya and West Nile DNA.
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<li>Our decision to focus on mosquitoes come from the significant challenges they pose in our region, making them a pressing concern and a prominent research subject. Numerous teams from different institutes have expressed their interest in this area, motivating us to develop a detection method that can streamline research efforts and contribute to the field.
Inspired by the work of our predecessors, we have adopted the SHERLOCK technique as our foundation. This approach utilizes Cas nucleases combined with a guide sequence that complements the viral RNA. When the viral sequence is identified, the Cas nuclease not only cleaves it but also cleaves the surrounding region, resulting in the release of a fluorescent molecule called FAM. Detection of FAM fluorescence serves as an indicator of viral presence.
In the initial phase, we embarked on a comparison between two Cas nucleases: the one employed by last year's team, Cas13a and a novel protein, CasRX, chosen by our own team. We evaluated their efficiency at various concentrations of viral sequences that we designed and had synthesized from Chikungunya and West Nile DNA.
<p>We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. Your Project Description should include more information than your project abstract.</p>