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2023 Competition
Manual-KY
Commits
8ac654e9
Commit
8ac654e9
authored
1 year ago
by
Vishwaa Kannan
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Final Patch experiments
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@@ -415,7 +415,7 @@
<li
style=
"list-style:disc;"
>
Centrifuge for an additional 1 minute and discard the flow through.
</li>
<li
style=
"list-style:disc;"
>
Transfer the column to a 1.5 mL microcentrifuge tube and add 50 μL of Elution Buffer
</li>
<li
style=
"list-style:disc;"
>
Centrifuge for 1 minute and store the flow-through safely
</li>
<ul>
<
/
ul>
<h3>
Ligation of Inserts
</h3>
<p>
Note: All numerical values of insert amount, plasmid amount, and buffer amount are based on our calculations
</p>
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