<listyle="list-style:disc;">Transfer the GeneJET column to a 1.5 mL microcentrifuge tube</li>
<listyle="list-style:disc;">Add 50 μL of the Elution Buffer and incubate at room temperature for 2 minutes</li>
<listyle="list-style:disc;">Centrifuge for 2 minutes and discard the column. Ensure that the flowthrough is stored safelyh</li>
<ul>
</ul>
<p>After nanodropping the plasmids from the miniprep, we decided to only use A11, C11, and E11 since, although G11 had high concentration, they had the highest purity (high 260/280 ratio).</p>