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Commit 90b2e0d6 authored by Srijyoth Ashok's avatar Srijyoth Ashok
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updated measurement

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---
layout: ../layouts/PageLayout.astro
title: Measurement
header: https://static.igem.wiki/teams/4683/wiki/header-images/microq.jpg
header: https://static.igem.wiki/teams/4683/wiki/header-images/screen-shot-2023-10-11-at-9-18-19-pm.png
---
import Wip from "../components/md_components/Wip";
......@@ -23,12 +23,13 @@ This year, we addressed these issues by developing Micro-Q Pro—a camera-based
## Fluorescence Background
Fluorescence is a type of luminescence that occurs when particular substances absorb light at one wavelength, and then emit light at a longer wavelength. When a fluorescent sample is exposed to a specific excitation wavelength, it absorbs the light energy, raising its molecules to higher energy states. As these excited molecules return to lower energy ground states, they release excess energy as fluorescent light (see Fig. 1).
Fluorescence is a type of luminescence that occurs when particular substances absorb light at one wavelength and then emit light at a longer wavelength. When a fluorescent sample is exposed to a specific excitation wavelength, it absorbs the light energy, raising its molecules to higher energy states. As these excited molecules return to lower energy ground states, they release excess energy as fluorescent light (see Fig. 1).
This fundamental process enables diverse applications for fluorescence, including bioimaging, medical diagnostics, forensics, and environmental monitoring. By tuning excitation light and detecting emission wavelengths, researchers can selectively visualize, quantify, and track fluorescent probes for many purposes.
<Image
src="https://static.igem.wiki/teams/4683/wiki/micro-q-figure-demonstrates-the-process-of-how-fluorescence-is-emitted-from-a-sample-2.png"
size=""
caption="Figure 1. The process of fluorescence excitation and emission from a sample."
/>
......@@ -61,7 +62,8 @@ Commercial spectrophotometers utilize white light lasers, which cover the full v
<Image
src="https://static.igem.wiki/teams/4683/wiki/measurement/fig3-2.png"
caption="Figure 3. Fluorescein sample excited at A) 30° B) 45° C) 60°; 45° showed least glare and best fluorescence."
caption="Figure 3. Fluorescein sample excited at A) 30° B) 45° C) 60°; 45° showed least glare and best fluorescence."
size: "350"
/>
### Fluorescence Quantification
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