<p>The firefly and renilla genes in the V2 vector were shown to be functioning.</p>
<divclass="image">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image7.png"alt="Dual Luciferase Assay of Putative Stable Cells Lines"style="width: 30%;"style="height: 30%">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image7.png"alt="Dual Luciferase Assay of Putative Stable Cells Lines"style="width: 70%;"style="height: 70%">
</div>
<p>Figure 5. Dual Luciferase Assay of Putative Stable Cells Lines</p>
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<p>The goal is to confirm the gene targets that, in cells, may be employed to prevent the formation of cholesterol and serve as the cornerstone for the creation of the V3 gene circuit. There are 17 enzymes that work together to catalyze the cholesterol production pathway in liver cells. We select the enzymes squalene monooxygenase (SQLE) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) based on previous research. In order to determine which of these genes are potential targets for the inhibitory mechanism of the gene and to assess the gene expression of these two enzymes, it was necessary to analyze the levels of expression of the genes encoding FDFT1 and SQLE in wild-type HEPG2 cells.</p>
<divclass="image">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image1.png"alt="SQLE Average ΔCq Values"style="width: 30%;"style="height: 30%">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image1.png"alt="SQLE Average ΔCq Values"style="width: 70%;"style="height: 70%">
</div>
<p>Figure 6. SQLE Average ΔCq Values</p>
<divclass="image">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image4.png"alt="FDFT1 Average ΔCq Values"style="width: 30%;"style="height: 30%">
<imgsrc="https://static.igem.wiki/teams/4896/wiki/image4.png"alt="FDFT1 Average ΔCq Values"style="width: 70%;"style="height: 70%">