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......@@ -16,7 +16,7 @@ engineering design cycle.{% endblock %}
<p><strong>WET LAB</strong></p>
<p>
In the lab, designing each minor experiment itself is an experience that takes one through the DBTL cycle. As
outlined in our Lab notebook, even staple molecular biology techniques like PCR and electroporation can prove to
outlined in our Lab Notebook, even staple molecular biology techniques like PCR and electroporation can prove to
be challenging when approaching it for the first time with new organisms and parts to test.
</p>
<p>
......@@ -95,9 +95,9 @@ engineering design cycle.{% endblock %}
<p>This thorough and systematic approach allowed us to gain a comprehensive understanding of the strengths and
weaknesses of each algorithm in the context of protein expression optimization. It also highlighted the
adaptability and robustness of the Genetic Algorithm in achieving the desired protein expression targets.</p>
<p>Our optimization algorithm was put to a final test by generating different RBS sequences for GFP expression in L.
lactis. This were then implemented in the wet lab. The results of this testing reflected a good differentiation
<p>Our optimization algorithm was put to a final test by generating different RBS sequences for GFP expression in <i>L.
lactis</i>. This were then implemented in the wet lab. The results of this testing reflected a good differentiation
between high and low expressing RBS variants. The results are described in detail under the 'Results' page.</p>
</div>
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......@@ -66,6 +66,6 @@ standardized genetic parts and systems in this beneficial chassis.{% endblock %}
</div>
<div style="margin-bottom: 50px;">
<img src="https://static.igem.wiki/teams/4931/wiki/application.svg" alt="Applications" class="img-fluid">
<img src="https://static.igem.wiki/teams/4931/wiki/applications.svg" alt="Applications" class="img-fluid">
</div>
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......@@ -13,11 +13,11 @@
</div>
<div class="Content" style="max-height: 100%;">
<p>
Safety has always been our team's foremost concern, and we have taken comprehensive measures to ensure a secure working environment. Our project revolves around <i>Lactococcus lactis</i>, a well-known microorganism, and the strain we utilized, MC1636, is non-pathogenic, falling within the biosafety level 1 classification and is a Generally Regarded as Safe (GRAS) organism. We underwent rigorous training, guided by experts from Dr. Guhan Jayaraman's lab, to handle Lactococcus lactis effectively.
Safety has always been our team's foremost concern, and we have taken comprehensive measures to ensure a secure working environment. Our project revolves around <i>Lactococcus lactis</i>, a well-known microorganism, and the strain we utilized, MC1636, is non-pathogenic, falling within the biosafety level 1 classification and is a Generally Regarded as Safe (GRAS) organism. We underwent rigorous training, guided by experts from Dr. Guhan Jayaraman's lab, to handle <i>Lactococcus lactis</i> effectively.
<br><br>
<u>Our safety measures included</u> :
<u>Our safety measures included</u>:
<br><br>
<b>Laboratory Infrastructure</b> : We conducted our research in a biosafety level 1 laboratory, which is well-equipped with essential facilities, including:
<b>Laboratory Infrastructure</b>: We conducted our research in a biosafety level 1 laboratory, which is well-equipped with essential facilities, including:
<ul>
<li class="list-element">A biosafety cabinet</li>
<li class="list-element">A shaking incubator</li>
......@@ -31,9 +31,9 @@
</ul>
</p>
<p>
<b>Sterile Conditions</b> : We maintained a sterile work environment by performing all tasks within the confines of a biosafety cabinet. This ensured that accidental contamination did not occur, particularly important since our work could have repercussions on adjacent labs.
<b>Sterile Conditions</b>: We maintained a sterile work environment by performing all tasks within the confines of a biosafety cabinet. This ensured that accidental contamination did not occur, particularly important since our work could have repercussions on adjacent labs.
<br><br>
<b>Safety Protocols</b> : To minimize risks, we diligently followed safety protocols, including:
<b>Safety Protocols</b>: To minimize risks, we diligently followed safety protocols, including:
<ul>
<li class="list-element">Wearing gloves and masks during all experiments.</li>
<li class="list-element">Practicing thorough handwashing before leaving the laboratory.</li>
......@@ -42,7 +42,7 @@
</ul>
</p>
<p>
In summary, our commitment to safety in handling Lactococcus lactis is unwavering, with strict adherence to established protocols and stringent measures to maintain a secure work environment.
In summary, our commitment to safety in handling <i>Lactococcus lactis</i> is unwavering, with strict adherence to established protocols and stringent measures to maintain a secure work environment.
</p>
</div>
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