Our proof of concept experiment consisted of experimentally determining extent of phytate breakdown in dough prepared using a millets, which have a high phytate content.<br>
We used the method described by Heinonen and Lahti (Anal Biochem 1981 May 15;113(2):313-7. doi: 10.1016/0003-2697(81)90082-8) to determine phytate breakdown. This method is used to estimate free inorganic phosphate, which is released when phytate is broken down. Unlike the well-known Fiske-Subbarow method of inorganic phosphate determination, phytate does not cause interference in the method of Heinonen and Lahti.<br>
We constructed a standard curve using KH2PO4 to establish the relationship between Pi and absorbance at 390 nm.<br>
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We constructed a standard curve using KH2PO4 to establish the relationship between Pi and absorbance at 390 nm.<br></p>