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<p>
We successfully demonstrated secretion with a relatively new type of tag called the VNp (Vesicle Nucleating peptide). We worked with <strong>34</strong> parts in total, contributing <strong>24 of them</strong> to the registry. Parts entered into the registry include the basic Biobrick parts for three distinct VNps, as well as composite parts that fuse phasin proteins to various secretion tags and reporters. Altogether, these parts focus on the secretion of PHB granules from E. coli. Parts are shown below.
We successfully demonstrated secretion with a relatively new type of tag called the VNp (Vesicle Nucleating
peptide). We worked with <strong>34</strong> parts in total, contributing <strong>24 of them</strong> to the
registry. Parts entered into the registry include the basic Biobrick parts for three distinct VNps, as well
as composite parts that fuse phasin proteins to various secretion tags and reporters. Altogether, these
parts focus on the secretion of PHB granules from E. coli. Parts are shown below.
</p>
<h2>Parts Used:</h2>
<table>
<tr>
<th>BioBrick</th>
<th>Part Name</th>
<th>Descriptions and Purpose</th>
<th>Status</th>
<th>Length(bp)</th>
</tr>
<tr>
<td>BBa_K4738000</td>
<td>Vesicle Nucleating peptide VNp2`</td>
<td>Basic, secretion tag</td>
<td>NEW, previously in literature</td>
<td>114</td>
</tr>
<tr>
<td>BBa_K4738001</td>
<td>Vesicle Nucleating peptide VNp6`</td>
<td>Basic, secretion tag</td>
<td>NEW, previously in literature</td>
<td>114</td>
</tr>
<tr>
<td>BBa_K4738002</td>
<td>Vesicle Nucleating peptide VNp15`</td>
<td>Basic, secretion tag</td>
<td>NEW, previously in literature</td>
<td>60</td>
</tr>
<tr>
<td>BBa_K2739004</td>
<td>Phasin (PhaP)</td>
<td>Basic, protein coding sequence</td>
<td>Existing</td>
<td>582</td>
</tr>
<tr>
<td>BBa_Z0251</td>
<td>T7 strong promoter</td>
<td>Basic, regulatory (promoter)</td>
<td>Existing</td>
<td>35</td>
</tr>
<tr>
<td>BBa_B0015</td>
<td>B0010-B0012 double terminator (T1 and T7 terminator respectively)</td>
<td>IDK, Terminator</td>
<td>Existing</td>
<td>129</td>
</tr>
<tr>
<td>BBa_K128005</td>
<td>6xHis affinity tag</td>
<td>Basic, tag</td>
<td>Existing</td>
<td>18</td>
</tr>
<tr>
<td>BBa_B0034</td>
<td>RBS-B0034, RBS based on Elowitz repressilator</td>
<td>Basic, RBS</td>
<td>Existing</td>
<td>12</td>
</tr>
<tr>
<td>pSB1A3</td>
<td>pSB1A3 plasmid backbone</td>
<td>Basic, backbone</td>
<td>Existing</td>
<td>2155</td>
</tr>
<tr>
<td>BBa_E0040*</td>
<td>GFP-mut3b</td>
<td>Basic, coding, reporter</td>
<td>Existing</td>
<td>720</td>
</tr>
<tr>
<td>BBa_E0040 (modified)</td>
<td>GFP-mut3b (removed start and stop codons to optimize for fusion protein)</td>
<td>Basic, coding, reporter</td>
<td>Existing</td>
<td>711</td>
</tr>
<tr>
<td>BBa_K3114015</td>
<td>Golden Gate Module Flipper</td>
<td>Device (idk if its basic or composite)</td>
<td>Existing</td>
<td>892</td>
</tr>
<tr>
<td>BBa_K1149051</td>
<td>Hybrid Promoter PhaCAB</td>
<td>Composite, PHB production</td>
<td>Existing</td>
<td>4271</td>
</tr>
<tr>
<td>BBa_K2549053</td>
<td>Gly-Gly-Gly-Gly-Ser linker</td>
<td>Basic, linker</td>
<td>Existing</td>
<td>15</td>
</tr>
<tr>
<td>BBa_K4738010</td>
<td>T7 - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>776</td>
</tr>
<tr>
<td>BBa_K4738011</td>
<td>T7 - His6 - PhaP - HlyA - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>962</td>
</tr>
<tr>
<td>BBa_K4738012</td>
<td>T7 - GeneIII - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>830</td>
</tr>
<tr>
<td>BBa_K4738013</td>
<td>T7 - TorA - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>914</td>
</tr>
<tr>
<td>BBa_K4738014</td>
<td>T7 - VNp2 - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>890</td>
</tr>
<tr>
<td>BBa_K4738015</td>
<td>T7 - VNp6 - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>890</td>
</tr>
<tr>
<td>BBa_K4738016</td>
<td>T7 - VNp15 - PhaP - His6 - T</td>
<td>Composite, Phasin secretion</td>
<td>Should be new</td>
<td>836</td>
</tr>
<tr>
<td>BBa_K4738020</td>
<td>T7 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1559</td>
</tr>
<tr>
<td>BBa_K4738021</td>
<td>T7 - His6 - PhaP - G4S - GFP(mut3b) - HlyA - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1711</td>
</tr>
<tr>
<td>BBa_K4738022</td>
<td>T7 - GeneIII - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1613</td>
</tr>
<tr>
<td>BBa_K4738023</td>
<td>T7 - TorA - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1698</td>
</tr>
<tr>
<td>BBa_K4738024</td>
<td>T7 - VNp2 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1673</td>
</tr>
<tr>
<td>BBa_K4738025</td>
<td>T7 - VNp6 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1673</td>
</tr>
<tr>
<td>BBa_K4738026</td>
<td>T7 - VNp15 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, Phasin-GFP fusion protein secretion</td>
<td>Should be new</td>
<td>1619</td>
</tr>
<tr>
<td>BBa_K4738030</td>
<td>Hybrid promoter PhaCAB - T7 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>5951</td>
</tr>
<tr>
<td>BBa_K4738031</td>
<td>Hybrid promoter PhaCAB - T7 - His6 - PhaP - G4S - GFP(mut3b) - HlyA - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6103</td>
</tr>
<tr>
<td>BBa_K4738032</td>
<td>Hybrid promoter PhaCAB - T7 - GeneIII - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6005</td>
</tr>
<tr>
<td>BBa_K4738033</td>
<td>Hybrid promoter PhaCAB - T7 - TorA - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6090</td>
</tr>
<tr>
<td>BBa_K4738034</td>
<td>Hybrid promoter PhaCAB - T7 - VNp2 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6065</td>
</tr>
<tr>
<td>BBa_K4738035</td>
<td>Hybrid promoter PhaCAB - T7 - VNp6 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6065</td>
</tr>
<tr>
<td>BBa_K4738036</td>
<td>Hybrid promoter PhaCAB - T7 - VNp15 - PhaP - G4S - GFP(mut3b) - His6 - T</td>
<td>Composite, PHB production</td>
<td>Should be new</td>
<td>6011</td>
</tr>
</table>
<table>
<tr>
<th>BioBrick</th>
<th>Part Name</th>
<th>Description and Purpose</th>
<th>Status</th>
<th>Length (bp)</th>
</tr>
<tr>
<td>BBa_K4738000</td>
<td>Human alpha-synuclein derived peptide VNp2 coding sequence</td>
<td>Basic, Secretion tag</td>
<td>NEW, previously in literature</td>
<td>114</td>
</tr>
<tr>
<td>BBa_K4738001</td>
<td>Human alpha-synuclein derived peptide VNp6 coding sequence</td>
<td>Basic, Secretion tag</td>
<td>NEW, previously in literature</td>
<td>114</td>
</tr>
<tr>
<td>BBa_K4738002</td>
<td>Human alpha-synuclein derived peptide VNp15 coding sequence</td>
<td>Basic, Secretion tag</td>
<td>NEW, previously in literature</td>
<td>60</td>
</tr>
<tr>
<td>BBa_K2739004</td>
<td>Phasin (PhaP)</td>
<td>Basic, Protein coding sequence</td>
<td>Existing</td>
<td>582</td>
</tr>
<tr>
<td>BBa_Z0251</td>
<td>T7 strong promoter</td>
<td>Basic, Constitutive promoter</td>
<td>Existing</td>
<td>35</td>
</tr>
<tr>
<td>BBa_B0015</td>
<td>B0010-B0012 T1-T7 double terminator</td>
<td>Basic, Terminator</td>
<td>Existing</td>
<td>129</td>
</tr>
<tr>
<td>BBa_K128005</td>
<td>6xHis affinity tag</td>
<td>Basic, Affinity tag</td>
<td>Existing</td>
<td>18</td>
</tr>
<tr>
<td>BBa_B0034</td>
<td>RBS-B0034, RBS based on Elowitz repressilator</td>
<td>Basic, RBS</td>
<td>Existing</td>
<td>12</td>
</tr>
<tr>
<td>pSB1A3</td>
<td>pSB1A3 plasmid backbone</td>
<td>Basic, backbone</td>
<td>Existing</td>
<td>2155</td>
</tr>
<tr>
<td>BBa_E0040</td>
<td>Wild-type GFP from Aequeora victoria</td>
<td>Basic, coding, reporter</td>
<td>Existing</td>
<td>720</td>
</tr>
<tr>
<td>BBa_K3114015</td>
<td>Golden Gate mRFP Module Flipper</td>
<td>Composite, Device</td>
<td>Existing</td>
<td>892</td>
</tr>
<tr>
<td>BBa_K1149051</td>
<td>Hybrid Promoter PhaCAB</td>
<td>Composite, PHB Production Construct</td>
<td>Existing</td>
<td>4271</td>
</tr>
<tr>
<td>BBa_K2549053</td>
<td>Gly-Gly-Gly-Gly-Ser linker</td>
<td>Basic, Flexible Linker</td>
<td>Existing</td>
<td>15</td>
</tr>
<tr>
<td>BBa_K4738010</td>
<td>PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>776</td>
</tr>
<tr>
<td>BBa_K4738011</td>
<td>His6-PhaP-HlyA fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>962</td>
</tr>
<tr>
<td>BBa_K4738012</td>
<td>GeneIII-PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>830</td>
</tr>
<tr>
<td>BBa_K4738013</td>
<td>TorA-PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>914</td>
</tr>
<tr>
<td>BBa_K4738014</td>
<td>VNp2-PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>890</td>
</tr>
<tr>
<td>BBa_K4738015</td>
<td>VNp6-PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>890</td>
</tr>
<tr>
<td>BBa_K4738016</td>
<td>VNp15-PhaP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin secretion device</td>
<td>NEW</td>
<td>836</td>
</tr>
<tr>
<td>BBa_K4738020</td>
<td>PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1511</td>
</tr>
<tr>
<td>BBa_K4738021</td>
<td>His6-PhaP-GFP-HlyA fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1697</td>
</tr>
<tr>
<td>BBa_K4738022</td>
<td>GeneIII-PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1565</td>
</tr>
<tr>
<td>BBa_K4738023</td>
<td>TorA-PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1649</td>
</tr>
<tr>
<td>BBa_K4738024</td>
<td>VNp2-PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1625</td>
</tr>
<tr>
<td>BBa_K4738025</td>
<td>VNp6-PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1625</td>
</tr>
<tr>
<td>BBa_K4738026</td>
<td>VNp15-PhaP-GFP-His6 fusion protein with T7 promoter</td>
<td>Composite, Phasin-GFP fusion protein secretion device</td>
<td>NEW</td>
<td>1571</td>
</tr>
<tr>
<td>BBa_K4738030</td>
<td>Hybrid PhaCAB composite w/PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5782</td>
</tr>
<tr>
<td>BBa_K4738031</td>
<td>Hybrid PhaCAB composite w/His6-PhaP-GFP-HlyA and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5968</td>
</tr>
<tr>
<td>BBa_K4738032</td>
<td>Hybrid PhaCAB w/GeneIII-PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5836</td>
</tr>
<tr>
<td>BBa_K4738033</td>
<td>Hybrid PhaCAB composite w/TorA-PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5920</td>
</tr>
<tr>
<td>BBa_K4738034</td>
<td>Hybrid PhaCAB composite w/VNp2-PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5896</td>
</tr>
<tr>
<td>BBa_K4738035</td>
<td>Hybrid PhaCAB composite w/VNp6-PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5896</td>
</tr>
<tr>
<td>BBa_K4738036</td>
<td>Hybrid PhaCAB composite w/VNp15-PhaP-GFP-His6 and T7 promoter</td>
<td>Composite, PHB production and secretion device</td>
<td>NEW</td>
<td>5842</td>
</tr>
</table>
</div>
</div>
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