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Commit f07c4ad5 authored by Devmc's avatar Devmc
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fix

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......@@ -72,7 +72,7 @@
<li>
Create the reaction system by adding the following reagents in their assigned portions into a microtube.
<table class="rw-100 m-t-1">
<table class="rw-75 mx-auto text-center m-t-1 table-head-bg-blue">
<tbody>
<tr>
<th>Reagent</th>
......@@ -94,16 +94,6 @@
<td>2μl</td>
<td>0.8μM</td>
</tr>
<tr>
<td>Loop F (10μM)</td>
<td>1μl</td>
<td>0.4μM</td>
</tr>
<tr>
<td>Loop B (10μM)</td>
<td>1μl</td>
<td>0.4μM</td>
</tr>
<tr>
<td>F3 (10μM)</td>
<td>0.5μl</td>
......@@ -116,7 +106,7 @@
</tr>
<tr>
<td>Template DNA</td>
<td>25-100 ng</td>
<td>1.5μl</td>
<td>1-4 ng/μl</td>
</tr>
<tr>
......@@ -126,20 +116,41 @@
</tr>
<tr>
<td>Sterilized ddH2O</td>
<td>Up to 25μl</td>
<td>5.5μl</td>
<td>-</td>
</tr>
</tbody>
</table>
</li>
<li>
Place the microtube into a 65C water bath and heat it for 30-60 minutes.
Place the microtube into a 65 water bath and heat it for 30-60 minutes.
</li>
<li>
Place the tube in a gradient thermo cycler and heat at 80C for 10 minutes to denature all DNA polymerase
Place the tube in a gradient thermo cycler and heat at 80 for 10 minutes to denature all DNA polymerase.
</li>
</ol>
<li>
Test resulting DNA using gel electrophoresis:
<ol type="a">
<li>
Prepare 1% gel electrophoresis by preparing the gel
</li>
<li>
Pipette 3-5μl of LAMP resultant using a pipette and run gel electrophoresis at 180V for 10 minutes.
</li>
<li>
Agarose electrophoresis was used for detection. Take 3-5μl amplification products, prepare 1% agar gel for
electrophoresis detection, and lamp characteristic maps composed of different size bands can be seen.
</li>
</ol>
</li>
</ol>
<div class="imager">
<img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/experiments/t-ykpao-experiments-01.jpg" alt="">
</div>
<div class="imager">
<img class="rw-100" src="https://static.igem.wiki/teams/4304/wiki/experiments/t-ykpao-experiments-02.jpg" alt="">
</div>
</div>
</div>
</section>
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