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2022 Competition
YkPaO
Commits
f07c4ad5
Commit
f07c4ad5
authored
2 years ago
by
Devmc
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fix
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ac458c21
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#128915
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2 years ago
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Stage: deploy
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wiki/experiments/section-9.html
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View file @
f07c4ad5
...
...
@@ -72,7 +72,7 @@
<li>
Create the reaction system by adding the following reagents in their assigned portions into a microtube.
<table
class=
"rw-
100 m-t-1
"
>
<table
class=
"rw-
75 mx-auto text-center m-t-1 table-head-bg-blue
"
>
<tbody>
<tr>
<th>
Reagent
</th>
...
...
@@ -94,16 +94,6 @@
<td>
2μl
</td>
<td>
0.8μM
</td>
</tr>
<tr>
<td>
Loop F (10μM)
</td>
<td>
1μl
</td>
<td>
0.4μM
</td>
</tr>
<tr>
<td>
Loop B (10μM)
</td>
<td>
1μl
</td>
<td>
0.4μM
</td>
</tr>
<tr>
<td>
F3 (10μM)
</td>
<td>
0.5μl
</td>
...
...
@@ -116,7 +106,7 @@
</tr>
<tr>
<td>
Template DNA
</td>
<td>
25-100 ng
</td>
<td>
1.5μl
</td>
<td>
1-4 ng/μl
</td>
</tr>
<tr>
...
...
@@ -126,20 +116,41 @@
</tr>
<tr>
<td>
Sterilized ddH2O
</td>
<td>
Up to 2
5μl
</td>
<td>
5.
5μl
</td>
<td>
-
</td>
</tr>
</tbody>
</table>
</li>
<li>
Place the microtube into a 65
C
water bath and heat it for 30-60 minutes.
Place the microtube into a 65
℃
water bath and heat it for 30-60 minutes.
</li>
<li>
Place the tube in a gradient thermo cycler and heat at 80
C
for 10 minutes to denature all DNA polymerase
Place the tube in a gradient thermo cycler and heat at 80
℃
for 10 minutes to denature all DNA polymerase
.
</li>
</ol>
<li>
Test resulting DNA using gel electrophoresis:
<ol
type=
"a"
>
<li>
Prepare 1% gel electrophoresis by preparing the gel
</li>
<li>
Pipette 3-5μl of LAMP resultant using a pipette and run gel electrophoresis at 180V for 10 minutes.
</li>
<li>
Agarose electrophoresis was used for detection. Take 3-5μl amplification products, prepare 1% agar gel for
electrophoresis detection, and lamp characteristic maps composed of different size bands can be seen.
</li>
</ol>
</li>
</ol>
<div
class=
"imager"
>
<img
class=
"rw-100"
src=
"https://static.igem.wiki/teams/4304/wiki/experiments/t-ykpao-experiments-01.jpg"
alt=
""
>
</div>
<div
class=
"imager"
>
<img
class=
"rw-100"
src=
"https://static.igem.wiki/teams/4304/wiki/experiments/t-ykpao-experiments-02.jpg"
alt=
""
>
</div>
</div>
</div>
</section>
\ No newline at end of file
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