<p>✓ We provide a new <i>pSB3CY</i>_shuttle vector backbone with aeBlue for <i>Saccharomyces cerevisiae</i> [<aclass="link-dark"href="http://parts.igem.org/Part:BBa_K4188001">BBa_K4188001</a>]</p>
<p>✓ We successfully designed, built, and tested a shuttle vector for S. cerevisiae transformations containing the 2µ ori and the chromoprotein aeBlue expression cassette. The latter is flanked by BsmBI restriction sites for easy introduction of a selection marker. Moreover, the shuttle vector includes the chromoprotein mRFP1 expression cassette between SapI restriction sites for insertion of a multi-transcription unit expressible in yeast.</br> Strains transformed with the newly designed level 2 shuttle vector can thus be selected by color. While the initial vector shows a purple color, successful transformants turn red if a selection marker is added via Golden Gate Assembly. Furthermore, the insertion of a successful Golden Gate Assembly with a multi-transcription unit into the site of the red chromoprotein (mRFP1) gene the vector shows blue staining of the colonies. If both chromoproteins are exchanged, the transformants become white and the vector can be used for transformation of S. cerevisiae after plasmid extraction.</p>
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<h3> Nominated for Best Wiki</h3>
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<p>✓ We provide RFC1000-compatible level 2 yeast shuttle vectors. Those have a shared design with the
RFC1000-compatible level 1 yeast shuttle vectors from iGEM Team TU Dresden. Using these shuttle vectors, an easy
introduction of all yeast-specific selection markers is possible. Moreover, they are compatible with the parts