<p>We engineered the yeast’s mevalonate pathway to overcome its rate-limiting steps. By introducing additional
heterologous genes and silencing endogenous genes, we modified the pathway to suit our needs. [<aclass="link-dark"
href="https://2022.igem.wiki/wwu-muenster/results#sccytosol">Cytosolic production of α-pinene in Saccharomyces
cerevisiae</a>] Our goal was to increase geranyl diphosphate (GPP) production in <i>Saccharomyces cerevisiae</i>, as it acts as an essential precursor for monoterpenoid synthesis such as α-pinene. Hence, we
heterologous genes and silencing endogenous genes, we <aclass="link-dark"
href="https://2022.igem.wiki/wwu-muenster/results#sccytosol">modified the pathway to suit our needs</a>. Our goal was to increase geranyl diphosphate (GPP) production in <i>Saccharomyces cerevisiae</i>, as it acts as an essential precursor for monoterpenoid synthesis such as α-pinene. Hence, we
engineered the cytosolic pathway, but also targeted another cell compartment. Since physiological conditions in
peroxisomes are favorable for GPP production, we hypothesize that an increased α-pinene concentration is observed if
the corresponding synthesis pathway is relocated there.</p>