<p>1. Test more enzymes such as P450, histine-ligated heme enzymes like TyrH and other hydroxylases or dehydrogenases</p>
<p>2. Understand what byrpoducts are formed after testing various enzymes and use this information to see if certain enzymes contribute to higher levels of toxicity from the creation of toxic byproducts or if there are safer enzymes that can partially degrade PFAS while also producing safe byproducts (get the best of both worlds)</p>
<p>3. Want to enginner our bacteria in Pseudomonas Putida (P. Putida) to test which type and strains of bacteria have a higher survival rate when degrading PFAS</p>
<p>Add a fluorescent tag to our insert will tell us if the protein is being produced. This guarantees a greater understanding of whether or not the promoter is working and helps us understand the expression of the gene</p>
<p>4. Protein Size (see if protein of expected size is there)
<p>4. Add a fluorescent tag to our insert will tell us if the protein is being produced. This guarantees a greater understanding of whether or not the promoter is working and helps us understand the expression of the gene</p>
<p>5. Protein Size (see if protein of expected size is there)
<pstyle="text-indent: 40px">a. Protein tag</p>
<pstyle="text-indent: 40px">b. Western blot</p>
<pstyle="text-indent: 40px">c. Fast Protein Liquid Chromatography (FPLC)</p>
