<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
<p>The Defluorinator project was created to remove PFOA from water systems. This can be done by breaking the carbon fluorine bond in PFOA which causes the chemical to break down. Two enzymes haloacid and fluoroacetate dehalogenase were chosen to break the fluorine bonds in PFOA. Plasmid was put into E. coli.</p>
<p>To determine if this sample was capable of defluorinating PFOA we measured the pH and optical density in 6 different cultures: E.Coli with engineered FaCD + PFOA, E. coli with engineered HaCD + PFOA, NEB5a E.coli + PFOA, and wild type P. putida + PFOA without plasmid. With two controls of engineered FaCD in e.coli, engineered HaCD in E.coli, and just LB broth. pH level and optical density were measured at several time points over 3 days or 54 hours (12:00 PM, 3:00 PM, 6:00 PM, 9:00 AM, 12:00 PM, 3:00 PM, 6:00 PM, 9:00 AM, 12:00 PM, 3:00 PM, 6:00 PM).</p>
