Wetlab update through 2pm oct 10

wiki/pages/notebook.html

@@ -1908,9 +1908,202 @@ Protocol</p>
   <button class="btn btn-primary btn-lg btn-block collapsed" data-bs-toggle="collapse" data-bs-target="#collapseThirtyseven" aria-expanded="false" aria-controls="collapseThirtyseven">Week 27 — Week of October 3</button>
     <div id="collapseThirtyseven" class="collapse" aria-labelledby="headingThirtyseven" data-bs-parent="#accordionFour">
       <div class="card-body">
-    <ul>
-      <li></li>
-    </ul>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 3rd</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  <li>Meghan Smith</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Performed golden gate reactions with the following combinations:</p>
+  <ol>
+<li>A linkers</li>
+<li>C linkers</li>
+<li>pSEVA3411</li>
+</ol>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed Golden Gate assembly protocol using a thermocycler</li>
+  </ol>
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 3rd</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  <li>Meghan Smith</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Performed golden gate reactions with the following combinations:</p>
+  <ol>
+<li>A linkers + UBQ - N</li>
+<li>C linkers + UBQ - C</li>
+<li>PSEVA 3411+ D-promoter</li>
+</ol>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed Golden Gate assembly protocol using a thermocycler</li>
+  </ol>
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 4th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Performed golden gate reactions with the following combinations:</p>
+  <ol>
+<li>Complementation fragments</li>
+<li>Ribo RBS</li>
+</ol>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed Golden Gate assembly protocol using a thermocycler</li>
+  </ol>
+<hr></hr>
+<h4>Lead: Meaghan Smith</h4>
+<h4>Date: October 6th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Meaghan Smith</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Completed pcr cleanup on golden gate samples:</p>
+  <ol>
+<li>A linkers + UBQ - N</li>
+<li>C linkers + UBQ - C</li>
+<li>Complementation systems + RiboJ complex</li>
+</ol>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed pcr cleanup protocol</li>
+  <li>Used 5:1 ratio for binding buffer & 15 μL elution buffer</li>
+  </ol>
+   <table class="table">
+  <thead class="thead-light">
+      <th scope="col">Combination</th>
+      <th scope="col">Nanodrop Concentration</th>
+      <th scope="col">260/280 Ratio</th>
+      <th scope="col">260/230 Ratio</th>
+  </thead>
+  <tbody>
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-alinkers-ubq-c.png">A linkers + UBQ - C</a></th>
+      <td class="text-center">141.45 ng/µL</td>      
+      <td class="text-center">1.843</td>
+      <td class="text-center">2.224</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-c-linkers-ubq-n.png">C linkers + UBQ - N</a></th>
+      <td class="text-center">125.10 ng/µL</td>      
+      <td class="text-center">1.841</td>
+      <td class="text-center">2.161</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-hrp.png">HRP</a></th>
+      <td class="text-center">159.80 ng/µL</td>      
+      <td class="text-center">1.839</td>
+      <td class="text-center">2.159</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-tev.png">TEV</a></th>
+      <td class="text-center">102.05 ng/µL</td>      
+      <td class="text-center">1.850</td>
+      <td class="text-center">2.288</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-blac.png">Blac</a></th>
+      <td class="text-center">104.00 ng/µL</td>      
+      <td class="text-center">1.851</td>
+      <td class="text-center">2.291</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-blac.png">Fluc</a></th>
+      <td class="text-center">128.9 ng/µL</td>      
+      <td class="text-center">1.871</td>
+      <td class="text-center">2.465</td>
+    </tr>     
+    <tr>
+      <th scope="row"><a href = "https://static.igem.wiki/teams/4215/wiki/10-6-rluc.png">Rluc</a></th>
+      <td class="text-center">130.2 ng/µL</td>      
+      <td class="text-center">1.849</td>
+      <td class="text-center">2.222</td>
+    </tr>                         
+  </tbody>
+</table> 
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 7th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Performed PCR to amplify Cassette A, B, and C</p>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed protocol for PCR using IGEM__GG (fwd and rev)</li>
+  </ol>
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 7th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  <li>Meaghan Smith</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Ran Gel electrophoresis on Complementation system cassette and then performed gel extraction to extract complete cassette.</p>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed protocol for gel electrophoresis and gel extraction.</li>
+  </ol>  
+<h4>Images:</h4>  
+<img src="https://static.igem.wiki/teams/4215/wiki/10-7-ladder.png" class = "img-fluid" alt="Gel from October 7 experiment compared to ladder">
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 8th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Amplified TEV gel extract</p>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed protocol for PCR using IGEM__GG (fwd and rev) on the extracted DNA from the gel ran on October 7th. Only ran PCR on TEV.</li>
+  </ol>  
+<hr></hr>
+<h4>Lead: Keane Deas</h4>
+<h4>Date: October 9th</h4>
+<h4>Participants</h4>
+<ul>
+  <li>Keane Deas</li>
+  </ul>
+  <h4>What did you do?</h4>
+<p>Cleaned DNA from PCR of TEV</p>
+<h4>How did you do it?</h4>
+<ol>
+  <li>Followed protocol for DNA cleanup using NEB PCR clean up kit.</li>
+  </ol>  
+   <table class="table">
+  <thead class="thead-light">
+      <th scope="col">Combination</th>
+      <th scope="col">Sample</th>
+      <th scope="col">260/280 Ratio</th>
+      <th scope="col">260/230 Ratio</th>
+  </thead>
+  <tbody>
+    <tr>
+      <th scope="row">TEV</th>
+      <td class="text-center">99.2 ng/µL</td>      
+      <td class="text-center">1.861</td>
+      <td class="text-center">2.645</td>
+    </tr>     
+  </tbody>
+</table>   
       </div>
     </div>
   </div>